A method for demonstrating the efficacy of interventions designed to limit infarct size following coronary occlusion: Beneficial effect of hyaluronidase

Lair G T Ribeiro, L. David Hillis, Eric K. Louie, Michael A. Davis, Peter R. Maroko, Eugene Braunwald

Research output: Contribution to journalArticle

Abstract

Summary: In order to measure the protective effect of interventions following coronary artery occlusions in dogs, the creatine kinase activity of myocardial tissue was assayed after 24 h and related to the myocardial blood flow of that tissue measured with 85Sr labelled microspheres injected 15 min after occlusion. This assay showed normal levels when flow exceeded 50 cm3·min-1·100 g-1. In myocardium with flow reduced to 0 to 15 cm3·min-1·100 g-1, creatine kinase activity was 7.6 ± 0.6 IU·mg-1 protein in control dogs and 13.1 ± 1.8 IU·mg-1 protein (P<0.01) in dogs given 500 NF units·kg-1 of hyaluronidase 20 min after occlusion. Where myocardial blood flow was reduced to 16 to 50cm3·min-1·100 g-1, creatine kinase activity was increased from 14.1 ± 1.1 to 20.5 ± 1.4 IU·mg-1 protein by hyaluronidase. This method therefore assesses ischaemic damage independent of electrophysiological measurements and confirms myocardial preservation by hyaluronidase.

Original languageEnglish (US)
Pages (from-to)334-340
Number of pages7
JournalCardiovascular Research
Volume12
Issue number6
DOIs
StatePublished - Jun 1978
Externally publishedYes

Fingerprint

Infarct Size
Hyaluronoglucosaminidase
Coronary Occlusion
Occlusion
Efficacy
Dogs
Blood Flow
Creatine Kinase
Proteins
Protein
Blood
Tissue
MB Form Creatine Kinase
Microspheres
Myocardium
Coronary Artery
Preservation
Assays
Coronary Vessels
Damage

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

A method for demonstrating the efficacy of interventions designed to limit infarct size following coronary occlusion : Beneficial effect of hyaluronidase. / Ribeiro, Lair G T; Hillis, L. David; Louie, Eric K.; Davis, Michael A.; Maroko, Peter R.; Braunwald, Eugene.

In: Cardiovascular Research, Vol. 12, No. 6, 06.1978, p. 334-340.

Research output: Contribution to journalArticle

Ribeiro, Lair G T ; Hillis, L. David ; Louie, Eric K. ; Davis, Michael A. ; Maroko, Peter R. ; Braunwald, Eugene. / A method for demonstrating the efficacy of interventions designed to limit infarct size following coronary occlusion : Beneficial effect of hyaluronidase. In: Cardiovascular Research. 1978 ; Vol. 12, No. 6. pp. 334-340.
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