Abstract
Summary: In order to measure the protective effect of interventions following coronary artery occlusions in dogs, the creatine kinase activity of myocardial tissue was assayed after 24 h and related to the myocardial blood flow of that tissue measured with 85Sr labelled microspheres injected 15 min after occlusion. This assay showed normal levels when flow exceeded 50 cm3·min-1·100 g-1. In myocardium with flow reduced to 0 to 15 cm3·min-1·100 g-1, creatine kinase activity was 7.6 ± 0.6 IU·mg-1 protein in control dogs and 13.1 ± 1.8 IU·mg-1 protein (P<0.01) in dogs given 500 NF units·kg-1 of hyaluronidase 20 min after occlusion. Where myocardial blood flow was reduced to 16 to 50cm3·min-1·100 g-1, creatine kinase activity was increased from 14.1 ± 1.1 to 20.5 ± 1.4 IU·mg-1 protein by hyaluronidase. This method therefore assesses ischaemic damage independent of electrophysiological measurements and confirms myocardial preservation by hyaluronidase.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 334-340 |
| Number of pages | 7 |
| Journal | Cardiovascular Research |
| Volume | 12 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1978 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Physiology (medical)
- Physiology
- Cardiology and Cardiovascular Medicine
Cite this
A method for demonstrating the efficacy of interventions designed to limit infarct size following coronary occlusion : Beneficial effect of hyaluronidase. / Ribeiro, Lair G T; Hillis, L. David; Louie, Eric K.; Davis, Michael A.; Maroko, Peter R.; Braunwald, Eugene.
In: Cardiovascular Research, Vol. 12, No. 6, 06.1978, p. 334-340.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A method for demonstrating the efficacy of interventions designed to limit infarct size following coronary occlusion
T2 - Beneficial effect of hyaluronidase
AU - Ribeiro, Lair G T
AU - Hillis, L. David
AU - Louie, Eric K.
AU - Davis, Michael A.
AU - Maroko, Peter R.
AU - Braunwald, Eugene
PY - 1978/6
Y1 - 1978/6
N2 - Summary: In order to measure the protective effect of interventions following coronary artery occlusions in dogs, the creatine kinase activity of myocardial tissue was assayed after 24 h and related to the myocardial blood flow of that tissue measured with 85Sr labelled microspheres injected 15 min after occlusion. This assay showed normal levels when flow exceeded 50 cm3·min-1·100 g-1. In myocardium with flow reduced to 0 to 15 cm3·min-1·100 g-1, creatine kinase activity was 7.6 ± 0.6 IU·mg-1 protein in control dogs and 13.1 ± 1.8 IU·mg-1 protein (P<0.01) in dogs given 500 NF units·kg-1 of hyaluronidase 20 min after occlusion. Where myocardial blood flow was reduced to 16 to 50cm3·min-1·100 g-1, creatine kinase activity was increased from 14.1 ± 1.1 to 20.5 ± 1.4 IU·mg-1 protein by hyaluronidase. This method therefore assesses ischaemic damage independent of electrophysiological measurements and confirms myocardial preservation by hyaluronidase.
AB - Summary: In order to measure the protective effect of interventions following coronary artery occlusions in dogs, the creatine kinase activity of myocardial tissue was assayed after 24 h and related to the myocardial blood flow of that tissue measured with 85Sr labelled microspheres injected 15 min after occlusion. This assay showed normal levels when flow exceeded 50 cm3·min-1·100 g-1. In myocardium with flow reduced to 0 to 15 cm3·min-1·100 g-1, creatine kinase activity was 7.6 ± 0.6 IU·mg-1 protein in control dogs and 13.1 ± 1.8 IU·mg-1 protein (P<0.01) in dogs given 500 NF units·kg-1 of hyaluronidase 20 min after occlusion. Where myocardial blood flow was reduced to 16 to 50cm3·min-1·100 g-1, creatine kinase activity was increased from 14.1 ± 1.1 to 20.5 ± 1.4 IU·mg-1 protein by hyaluronidase. This method therefore assesses ischaemic damage independent of electrophysiological measurements and confirms myocardial preservation by hyaluronidase.
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UR - http://www.scopus.com/inward/citedby.url?scp=0018075846&partnerID=8YFLogxK
U2 - 10.1093/cvrese/12.6.334
DO - 10.1093/cvrese/12.6.334
M3 - Article
C2 - 698985
VL - 12
SP - 334
EP - 340
JO - Cardiovascular Research
JF - Cardiovascular Research
SN - 0008-6363
IS - 6
ER -