A Lipidomics View of Circadian Biology

Rona Aviram, Chunyan Wang, Xianlin Han, Gad Asher

Research output: Contribution to journalArticlepeer-review

Abstract

Lipidomics approaches provide quantitative characterization of hundreds of lipid species from biological samples. Recent studies highlight the value of these methods in studying circadian biology, and their potential goes far beyond studying lipid metabolism per se. For example, lipidomics analyses of subcellular compartments can be used to determine daily rhythmicity of different organelles and their intracellular dynamics. In this chapter we describe in detail the procedure for around the clock shotgun lipidomics, from sample preparation to bioinformatics analyses. Sample preparation includes biochemical fractionation of nuclei and mitochondria from mouse liver harvested throughout the day. Lipid content is determined and quantified, in unbiased manner and with wide coverage, using multidimensional mass spectrometry shotgun lipidomics (MDMS-SL). Circadian parameters are then determined with nonparametric statistical tests. Overall, the approach described herein is applicable for various animal models, tissues, and organelles, and is expected to yield new insight on various aspects of circadian biology and lipid metabolism.

Original languageEnglish (US)
Pages (from-to)157-168
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
Volume2130
DOIs
StatePublished - 2021

Keywords

  • Circadian
  • Lipid metabolism
  • Liver
  • Mass spectrometry
  • Mitochondria
  • Mouse
  • Nuclei
  • Shotgun lipidomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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