A large indel mutation of the bovine ADD1/SREBP1c gene and its effects on growth traits in some native cattle breeds from China

Yong Zhen Huang, En Ping Zhang, Jing Wang, Yong Tao Huai, Liang Ma, Fu Ying Chen, Xian Yong Lan, Chu Zhao Lei, Xing Tang Fang, Ju Qiang Wang, Hong Chen

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Adipocyte determination and differentiation-dependent factor 1/sterol regulatory element-binding protein-1c (ADD1/SREBP1c) is a major determinant of tissue differential lipogenic capacity in mammalian and avian species. The objectives of the present study were to focus on insertion-deletion polymorphism (indel) in the bovine ADD1/SREBP1c gene, and analyzing its effect on growth traits in a sample of 1035 cattle belonging to four Chinese cattle breeds. PCR-SSCP, DNA sequencing and agarose electrophoresis methods were used. The 778 bp PCR products of ADD1/SREBP1c gene exhibited three genotypes and two alleles were revealed: W and D. Frequencies of the W allele varied from 0.8651 to 1.000. The associations of the 84 bp indel mutation of ADD1/SREBP1c gene with growth traits of 265 Nanyang cows were analyzed. The animals with genotype WD had significantly higher birth weight, body weight, average daily gain than those with genotype WW at birth, 6-, 12-, 18-, and 24-month old (P < 0.05 or P < 0.01). These results suggest that the indel mutation of bovine ADD1/SREBP1c gene may influence the growth traits in cattle.

Original languageEnglish (US)
Pages (from-to)2037-2042
Number of pages6
JournalMolecular Biology Reports
Volume38
Issue number3
DOIs
StatePublished - Mar 2011
Externally publishedYes

Keywords

  • ADD1/SREBP1c gene
  • Cattle
  • Growth traits
  • Indel mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'A large indel mutation of the bovine ADD1/SREBP1c gene and its effects on growth traits in some native cattle breeds from China'. Together they form a unique fingerprint.

Cite this