A-kinase anchoring protein 79/150 coordinates metabotropic glutamate receptor sensitization of peripheral sensory neurons

Kalina Szteyn, Matthew P. Rowan, Ruben Gomez, Junhui Du, Susan M. Carlton, Nathaniel Jeske

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Glutamate serves as the primary excitatory neurotransmitter in the nervous system. Previous studies have identified a role for glutamate and group I metabotropic receptors as targets for study in peripheral inflammatory pain. However, the coordination of signaling events that transpire from receptor activation to afferent neuronal sensitization has not been explored. Herein, we identify that scaffolding protein A-kinase anchoring protein 79/150 (AKAP150) coordinates increased peripheral thermal sensitivity after group I metabotropic receptor (mGluR5) activation. In both acute and persistent models of thermal somatosensory behavior, we report that mGluR5 sensitization requires AKAP150 expression. Furthermore, electrophysiological approaches designed to record afferent neuronal activity reveal that mGluR5 sensitization also requires functional AKAP150 expression. In dissociated primary afferent neurons, mGluR5 activation increases TRPV1 responses in an AKAP-dependent manner through a mechanism that induces AKAP association with TRPV1. Experimental results presented herein identify a mechanism of receptor-driven scaffolding association with ion channel targets. Importantly, this mechanism could prove significant in the search for therapeutic targets that repress episodes of acute pain from becoming chronic in nature.

Original languageEnglish (US)
Pages (from-to)2364-2372
Number of pages9
JournalPain
Volume156
Issue number11
DOIs
StatePublished - Nov 1 2015

Fingerprint

Metabotropic Glutamate Receptors
Sensory Receptor Cells
Protein Kinases
Glutamic Acid
Hot Temperature
Afferent Neurons
Staphylococcal Protein A
Acute Pain
Ion Channels
Nervous System
Neurotransmitter Agents
Pain
Proteins
Therapeutics

Keywords

  • AKAP
  • Glutamate
  • Peripheral
  • Skin-nerve prep
  • TRPV1

ASJC Scopus subject areas

  • Clinical Neurology
  • Anesthesiology and Pain Medicine
  • Neurology
  • Pharmacology

Cite this

A-kinase anchoring protein 79/150 coordinates metabotropic glutamate receptor sensitization of peripheral sensory neurons. / Szteyn, Kalina; Rowan, Matthew P.; Gomez, Ruben; Du, Junhui; Carlton, Susan M.; Jeske, Nathaniel.

In: Pain, Vol. 156, No. 11, 01.11.2015, p. 2364-2372.

Research output: Contribution to journalArticle

Szteyn, Kalina ; Rowan, Matthew P. ; Gomez, Ruben ; Du, Junhui ; Carlton, Susan M. ; Jeske, Nathaniel. / A-kinase anchoring protein 79/150 coordinates metabotropic glutamate receptor sensitization of peripheral sensory neurons. In: Pain. 2015 ; Vol. 156, No. 11. pp. 2364-2372.
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