A-kinase anchoring protein 150 mediates transient receptor potential family V type 1 sensitivity to phosphatidylinositol-4,5-bisphosphate

Nathaniel Jeske, Elaine D. Por, Sergei Belugin, Sraboni Chaudhury, Kelly A Berg, Armen N Akopian, Michael A. Henry, Ruben Gomez

Research output: Contribution to journalArticle

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Abstract

A-kinase anchoring protein 150 (AKAP150) is a scaffolding protein that controls protein kinase A- and C-mediated phosphorylation of the transient receptor potential family V type 1 (TRPV1), dictating receptor response to nociceptive stimuli. The phospholipid phosphatidylinositol-4,5-bisphosphate (PIP2) anchors AKAP150 to the plasma membrane in naive conditions and also affects TRPV1 activity. In the present study, we sought to determine whether the effects of PIP2 on TRPV1 are mediated through AKAP150. In trigeminal neurons and CHO cells, the manipulation of cellular PIP2 led to significant changes in the association of AKAP150 and TRPV1. Following PIP2 degradation, increased TRPV1: AKAP150 coimmunoprecipitation was observed, resulting in increased receptor response to capsaicin treatment. Phospholipase C activation in neurons isolated from AKAP150 animals indicated that PIP2-mediated inhibition of TRPV1 in the whole-cell environment requires expression of the scaffolding protein. Furthermore, the addition of PIP2 to neurons isolated from AKAP150 wild-type mice reduced PKA sensitization of TRPV1 compared with isolated neurons from AKAP150 mice. These findings suggest that PIP2 degradation increases AKAP150 association with TRPV1 in the whole-cell environment, leading to sensitization of the receptor to nociceptive stimuli.

Original languageEnglish (US)
Pages (from-to)8681-8688
Number of pages8
JournalJournal of Neuroscience
Volume31
Issue number23
DOIs
StatePublished - Jun 8 2011

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Phosphatidylinositols
Protein Kinases
Neurons
Cyclic AMP-Dependent Protein Kinase Type I
CHO Cells
Capsaicin
Type C Phospholipases
Cyclic AMP-Dependent Protein Kinases
Protein Kinase C
Phospholipids
Proteins
Phosphorylation
Cell Membrane

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

A-kinase anchoring protein 150 mediates transient receptor potential family V type 1 sensitivity to phosphatidylinositol-4,5-bisphosphate. / Jeske, Nathaniel; Por, Elaine D.; Belugin, Sergei; Chaudhury, Sraboni; Berg, Kelly A; Akopian, Armen N; Henry, Michael A.; Gomez, Ruben.

In: Journal of Neuroscience, Vol. 31, No. 23, 08.06.2011, p. 8681-8688.

Research output: Contribution to journalArticle

Jeske, Nathaniel ; Por, Elaine D. ; Belugin, Sergei ; Chaudhury, Sraboni ; Berg, Kelly A ; Akopian, Armen N ; Henry, Michael A. ; Gomez, Ruben. / A-kinase anchoring protein 150 mediates transient receptor potential family V type 1 sensitivity to phosphatidylinositol-4,5-bisphosphate. In: Journal of Neuroscience. 2011 ; Vol. 31, No. 23. pp. 8681-8688.
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