A hybrid quantum dot - Antibody fragment fluorescence resonance energy transfer-based TNT sensor

Ellen R. Goldman, Igor L. Medintz, Jessica L. Whitley, Andrew Hayhurst, Aaron R. Clapp, H. Tetsuo Uyeda, Jeffrey R. Deschamps, Michael E. Lassman, Hedi Mattoussi

    Research output: Contribution to journalArticlepeer-review

    508 Scopus citations

    Abstract

    We demonstrate the use of luminescent QDs conjugated to antibody fragments to develop solution-phase nanoscale sensing assemblies, based on fluorescence resonance energy transfer (FRET) for the specific detection of the explosive 2,4,6-trinitrotoluene (TNT) in aqueous environments. The hybrid sensor consists of anti-TNT specific antibody fragments attached to a hydrophilic QD via metal-affinity coordination. A dye-labeled TNT analogue prebound in the antibody binding site quenches the QD photoluminescence via proximity-induced FRET. Analysis of the data collected at increasing dye-labeled analogue to QD ratios provided an insight into understanding how the antibody fragments self-assemble on the QD. Addition of soluble TNT displaces the dye-labeled analogue, eliminating FRET and resulting in a concentration-dependent recovery of QD photoluminescence. Sensor performance and specificity were evaluated.

    Original languageEnglish (US)
    Pages (from-to)6744-6751
    Number of pages8
    JournalJournal of the American Chemical Society
    Volume127
    Issue number18
    DOIs
    StatePublished - May 11 2005

    ASJC Scopus subject areas

    • Catalysis
    • Chemistry(all)
    • Biochemistry
    • Colloid and Surface Chemistry

    Fingerprint Dive into the research topics of 'A hybrid quantum dot - Antibody fragment fluorescence resonance energy transfer-based TNT sensor'. Together they form a unique fingerprint.

    Cite this