A human (3.3kb) haptoglobin-cat transgene is modulated in lungs of transgenic mice by inflammation

Andrew Martinez, Laila Jansen, James M. Buchanan, Gwen S. Adrian, Fun Mei Yang, Damon C. Herbert, Frank J. Weaker, Christi A. Walter, Barbara H. Bowman

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Four independent lines of transgenic mice were produced carrying integrated copies of a chimeric gene composed of 3.3 kb of the human haptoglobin 5′ regulatory region fused to the CAT (chloramphenicol acetyl transferase) reporter gene. Although the endogenous mouse haptoglobin (Hp) and human haptoglobin (HP) genes express mainly in liver and lung, expression of the human 3.3-kb HP-CAT transgene was not detected until after induction of inflammation and then only in lungs. The results indicated that the transgene maintained the regulatory DNA elements required for lung specific responsiveness to inflammation in vivo but lacked the DNA sequence required for robust expression in liver. The DNA sequence(s) responsible for the normally high level of HP expression in liver either reside outside the 3.3-kb regulatory region of the HP chimeric gene or this region contains a suppressor sequence affecting tissue specific expression in the liver.

Original languageEnglish (US)
Pages (from-to)309-315
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume208
Issue number1
DOIs
StatePublished - Mar 8 1995

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Martinez, A., Jansen, L., Buchanan, J. M., Adrian, G. S., Yang, F. M., Herbert, D. C., Weaker, F. J., Walter, C. A., & Bowman, B. H. (1995). A human (3.3kb) haptoglobin-cat transgene is modulated in lungs of transgenic mice by inflammation. Biochemical and Biophysical Research Communications, 208(1), 309-315. https://doi.org/10.1006/bbrc.1995.1339