A hierarchy of T cell receptor motifs determines responsiveness to the immunodominant epitope in experimental autoimmune myasthenia gravis

Nathan E. Standifer; Ellen Kraig; Anthony J. Infante

doi:10.1016/j.jneuroim.2003.09.007

A hierarchy of T cell receptor motifs determines responsiveness to the immunodominant epitope in experimental autoimmune myasthenia gravis

Nathan E. Standifer, Ellen Kraig, Anthony J. Infante

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The predominant murine T lymphocyte population responding to Tα146-162, the immunodominant epitope in EAMG, expresses the TCRBV 6 gene segment. However, cells expressing other TCRBV gene segments also react with this peptide. In order to more precisely characterize the Tα146-162- specific TCR repertoire, we isolated CD4^high cells from peptide-immunized mice. The majority of CD4^high cells utilized an acidic TCR β chain CDR3 motif regardless of TCRBV gene usage. Analysis of T cell clones demonstrated a fourfold higher avidity of Vβ6+ than non-Vβ6 cells for Tα146-162 indicating that a hierarchy of TCR motifs determines T cell responsiveness in EAMG.

Original language	English (US)
Pages (from-to)	68-76
Number of pages	9
Journal	Journal of Neuroimmunology
Volume	145
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2003.09.007
State	Published - Dec 2003

Keywords

Avidity
Myasthenia gravis
T cell receptor
T lymphocytes

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/j.jneuroim.2003.09.007

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@article{54f488a5d6f641b4afeb9383116323d6,

title = "A hierarchy of T cell receptor motifs determines responsiveness to the immunodominant epitope in experimental autoimmune myasthenia gravis",

abstract = "The predominant murine T lymphocyte population responding to Tα146-162, the immunodominant epitope in EAMG, expresses the TCRBV 6 gene segment. However, cells expressing other TCRBV gene segments also react with this peptide. In order to more precisely characterize the Tα146-162- specific TCR repertoire, we isolated CD4high cells from peptide-immunized mice. The majority of CD4high cells utilized an acidic TCR β chain CDR3 motif regardless of TCRBV gene usage. Analysis of T cell clones demonstrated a fourfold higher avidity of Vβ6+ than non-Vβ6 cells for Tα146-162 indicating that a hierarchy of TCR motifs determines T cell responsiveness in EAMG.",

keywords = "Avidity, Myasthenia gravis, T cell receptor, T lymphocytes",

author = "Standifer, {Nathan E.} and Ellen Kraig and Infante, {Anthony J.}",

note = "Funding Information: The authors wish to thank Charles A. Thomas III of the University of Texas Health Science Center Institutional Flow Cytometry Core Facility for technical expertise in cell-sorting experiments, Steve Mouton for amino acid analyses of peptides, and Patricia Currier for technical expertise in T cell cloning. This work was supported by Grant AI43061 (A.J.I.) from the NIH.",

year = "2003",

month = dec,

doi = "10.1016/j.jneuroim.2003.09.007",

language = "English (US)",

volume = "145",

pages = "68--76",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier",

number = "1-2",

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TY - JOUR

T1 - A hierarchy of T cell receptor motifs determines responsiveness to the immunodominant epitope in experimental autoimmune myasthenia gravis

AU - Standifer, Nathan E.

AU - Kraig, Ellen

AU - Infante, Anthony J.

N1 - Funding Information: The authors wish to thank Charles A. Thomas III of the University of Texas Health Science Center Institutional Flow Cytometry Core Facility for technical expertise in cell-sorting experiments, Steve Mouton for amino acid analyses of peptides, and Patricia Currier for technical expertise in T cell cloning. This work was supported by Grant AI43061 (A.J.I.) from the NIH.

PY - 2003/12

Y1 - 2003/12

N2 - The predominant murine T lymphocyte population responding to Tα146-162, the immunodominant epitope in EAMG, expresses the TCRBV 6 gene segment. However, cells expressing other TCRBV gene segments also react with this peptide. In order to more precisely characterize the Tα146-162- specific TCR repertoire, we isolated CD4high cells from peptide-immunized mice. The majority of CD4high cells utilized an acidic TCR β chain CDR3 motif regardless of TCRBV gene usage. Analysis of T cell clones demonstrated a fourfold higher avidity of Vβ6+ than non-Vβ6 cells for Tα146-162 indicating that a hierarchy of TCR motifs determines T cell responsiveness in EAMG.

AB - The predominant murine T lymphocyte population responding to Tα146-162, the immunodominant epitope in EAMG, expresses the TCRBV 6 gene segment. However, cells expressing other TCRBV gene segments also react with this peptide. In order to more precisely characterize the Tα146-162- specific TCR repertoire, we isolated CD4high cells from peptide-immunized mice. The majority of CD4high cells utilized an acidic TCR β chain CDR3 motif regardless of TCRBV gene usage. Analysis of T cell clones demonstrated a fourfold higher avidity of Vβ6+ than non-Vβ6 cells for Tα146-162 indicating that a hierarchy of TCR motifs determines T cell responsiveness in EAMG.

KW - Avidity

KW - Myasthenia gravis

KW - T cell receptor

KW - T lymphocytes

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AN - SCOPUS:0344872721

SN - 0165-5728

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JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 1-2

ER -

A hierarchy of T cell receptor motifs determines responsiveness to the immunodominant epitope in experimental autoimmune myasthenia gravis

Abstract

Keywords

ASJC Scopus subject areas

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