A genome search identifies major quantitative trait loci on human chromosomes 3 and 4 that influence cholesterol concentrations in small LDL particles

David L. Rainwater, Laura Almasy, John Blangero, Shelley A. Cole, John L. VandeBerg, Jean W. MacCluer, James E. Hixson

Research output: Contribution to journalArticle

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Abstract

Small, dense LDL particles are associated with increased risk of cardiovascular disease. To identify the genes that influence LDL size variation, we performed a genome-wide screen for cholesterol concentrations in 4 LDL size fractions. Samples from 470 members of randomly ascertained families were typed for 331 microsatellite markers spaced at ≃ 15 cM intervals. Plasma LDLs were resolved by using nondenaturing gradient gel electrophoresis into 4 fraction sizes (LDL-1, 26.4 to 29.0 nm; LDL-2, 25.5 to 26.4 nm; LDL-3, 24.2 to 25.5 nm; and LDL-4, 21.0 to 24.2 nm) and cholesterol concentrations were estimated by staining with Sudan Black B. Linkage analyses used variance component methods that exploited all of the genotypic and phenotypic information in the large extended pedigrees. In multipoint linkage analyses with quantitative trait loci for the 4 fraction sizes, only LDL-3, a fraction containing small LDL particles, gave peak multipoint log10 odds in favor of linkage (LOD) scores that exceeded 3.0, a nominal criterion for evidence of significant linkage. The highest LOD scores for LDL-3 were found on chromosomes 3 (LOD=4.1), 4 (LOD=4.1), and 6 (LOD=2.9). In oligogenic analyses, the 2-locus LOD score (for chromosomes 3 and 4) increased significantly (P=0.0012) to 6.1, but including the third locus on chromosome 6 did not significantly improve the LOD score (P=0.064). Thus, we have localized 2 major quantitative trait loci that influence variation in cholesterol concentrations of small LDL particles. The 2 quantitative trait loci on chromosomes 3 and 4 are located in regions that contain the genes for apoD and the large subunit of the microsomal triglyceride transfer protein, respectively.

Original languageEnglish (US)
Pages (from-to)777-783
Number of pages7
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume19
Issue number3
StatePublished - Mar 1999
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 4
Chromosomes, Human, Pair 3
Quantitative Trait Loci
Human Chromosomes
Cholesterol
Genome
LDL Lipoproteins
oxidized low density lipoprotein
low density lipoprotein inhibitor
Chromosomes, Human, Pair 6
Pedigree
Microsatellite Repeats
Genes
Electrophoresis
Analysis of Variance
Cardiovascular Diseases
Gels
Staining and Labeling

Keywords

  • Genome-wide screen
  • LDL size fractions
  • Linkage analysis
  • Mexican Americans

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Rainwater, D. L., Almasy, L., Blangero, J., Cole, S. A., VandeBerg, J. L., MacCluer, J. W., & Hixson, J. E. (1999). A genome search identifies major quantitative trait loci on human chromosomes 3 and 4 that influence cholesterol concentrations in small LDL particles. Arteriosclerosis, Thrombosis, and Vascular Biology, 19(3), 777-783.

A genome search identifies major quantitative trait loci on human chromosomes 3 and 4 that influence cholesterol concentrations in small LDL particles. / Rainwater, David L.; Almasy, Laura; Blangero, John; Cole, Shelley A.; VandeBerg, John L.; MacCluer, Jean W.; Hixson, James E.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 19, No. 3, 03.1999, p. 777-783.

Research output: Contribution to journalArticle

Rainwater, David L. ; Almasy, Laura ; Blangero, John ; Cole, Shelley A. ; VandeBerg, John L. ; MacCluer, Jean W. ; Hixson, James E. / A genome search identifies major quantitative trait loci on human chromosomes 3 and 4 that influence cholesterol concentrations in small LDL particles. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 1999 ; Vol. 19, No. 3. pp. 777-783.
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