A genetic analysis of serotonergic biosynthetic and metabolic enzymes in migraine using a DNA pooling approach

Matthew P. Johnson, Lyn R. Griffiths

    Research output: Contribution to journalArticle

    22 Scopus citations

    Abstract

    Migraine is a common debilitating primary headache disorder with significant mental, physical and social health implications. The brain neurotransmitter 5-hydroxytryptamine (5-HT; serotonin) is involved in nociceptive pathways and has been implicated in the pathophysiology of migraine. With few genetic studies investigating biosynthetic and metabolic enzymes governing the rate of 5-HT activity and their relationship to migraine, it was the objective of this study to assess genetic variants within the human tryptophan hydroxylase (TPH), amino acid decarboxylase (AADC) and monoamine oxidase A (MAOA) genes in migraine susceptibility. This objective was undertaken using a high-throughput DNA pooling experimental design, which proved to be a very accurate, sensitive and specific method of estimating allele frequencies for single nucleotide polymorphism, insertion deletion and variable number tandem repeat loci. Application of DNA pooling to a wide array of genetic loci provides greater scope in the assessment of population-based genetic association study designs. Despite the application of this high-throughput genotyping method, negative results from the two-stage DNA pooling design used to screen loci within the TPH, AADC and MAOA genes did not support their role in migraine susceptibility.

    Original languageEnglish (US)
    Pages (from-to)607-610
    Number of pages4
    JournalJournal of Human Genetics
    Volume50
    Issue number12
    DOIs
    StatePublished - Dec 1 2005

    Keywords

    • DNA pooling
    • Genetic association
    • Migraine
    • Serotonin

    ASJC Scopus subject areas

    • Genetics
    • Genetics(clinical)

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