A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast

Robert J. Klebe, June V. Harriss, Z. Dave Sharp, Michael G. Douglas

Research output: Contribution to journalArticlepeer-review

364 Scopus citations

Abstract

Polyethylene glycol (PEG) can induce genetic transformation in both bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) without cell wall removal. PEG-mediated transformation of E. coli is technically simple and yields transformants with an efficiency of 106-107 transformants/μg DNA. Detailed analysis of the parameters involved in PEG-mediated transformation of E. coli reveals basic differences between the PEG and standard CaCl2 methods for transformation of E. coli. PEG-mediated transformation of yeast is far simpler than existing protoplast methods and is comparable in efficiency. The new methods described here for PEG-mediated genetic transformation may prove to be of general utility in performing genetic transformation in a wide variety of organisms.

Original languageEnglish (US)
Pages (from-to)333-341
Number of pages9
JournalGene
Volume25
Issue number2-3
DOIs
StatePublished - Nov 1983

Keywords

  • CaCl
  • Escherichia coli
  • Saccharomyces cerevisiae
  • Transformation
  • polyethylene glycol
  • protoplasts
  • recombinant DNA

ASJC Scopus subject areas

  • Genetics

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