A gene-specific promoter in transgenic mice directs testis-specific demethylation prior to transcriptional activation in vivo

L. Peter Zhang, James C. Stroud, Christi A. Walter, Gwendolyn S. Adrian, John R. McCarrey

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Transcription of the autosomal phosphoglycerate kinase gene, Pgk-2, is initiated at the onset of meiosis during spermatogenesis in mammals. However, in the mouse, the 5' portion of the endogenous Pgk-2 coding sequence undergoes a specific demethylation event that precedes transcriptional activation by 10-12 days. Here we show that transgenes consisting of the Pgk2 core promoter ligated to the CAT reporter gene undergo a similar tissue-, stage-, and cell type-specific demethylation in the 5' portion of the CAT coding sequence, whereas transgenes consisting of the CAT reporter sequence alone, or of the CAT sequence ligated to the CpG island-containing transferrin gene promoter, demonstrate different patterns of demethylation. These results indicate that specific promoter sequences can influence the pattern of tissue-specific demethylation within different genes and that a signal for spermatogenic cell-specific demethylation resides within the core promoter of the mammalian Pgk-2 gene.

Original languageEnglish (US)
Pages (from-to)284-292
Number of pages9
JournalBiology of reproduction
Volume59
Issue number2
DOIs
StatePublished - Aug 1998

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

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