A gene activated in mouse 3T3 cells by serum growth factors encodes a protein with 'zinc finger' sequences

B. A. Christy, L. F. Lau, D. Nathans

Research output: Contribution to journalArticle

548 Citations (Scopus)

Abstract

We have recently identified by cDNA cloning a set of genes that are rapidly activated in mouse 3T3 cells by serum or purified growth factors. Here we report that the cDNA (clone 268) derived from one of these immediate early genes (zif/268) encodes a protein with three tandem 'zinc finger' sequences typical of a class of eukaryotic transcription factors. The mRNA of zif/268 is present in many organs and tissues of the mouse and is especially abundant in the brain and thymus tissue. The 5' genomic flanking sequence of zif/268 has sequences related to binding sites for known regulatory proteins, including four sequences that resemble the core of the serum response elements (SREs) upstream of the c-fos and actin genes. The SRE-like sequences could be responsible for the coordinate activation of zif/268 and fos after serum stimulation of 3T3 cells.

Original languageEnglish (US)
Pages (from-to)7857-7861
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number21
StatePublished - 1988
Externally publishedYes

Fingerprint

Serum Response Element
3T3 Cells
Zinc Fingers
Intercellular Signaling Peptides and Proteins
Complementary DNA
fos Genes
Immediate-Early Genes
5' Flanking Region
Serum
Thymus Gland
Genes
Actins
Organism Cloning
Proteins
Transcription Factors
Clone Cells
Binding Sites
Messenger RNA
Brain

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

A gene activated in mouse 3T3 cells by serum growth factors encodes a protein with 'zinc finger' sequences. / Christy, B. A.; Lau, L. F.; Nathans, D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 21, 1988, p. 7857-7861.

Research output: Contribution to journalArticle

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