Abstract
The major isozyme of alcohol dehydrogenase in baboon stomach, ADH3, has been purified to homogeneity and characterized with a range of alcohol and aldehyde substrates. Using Kcat/Km, values as an indication of substrate efficacy, medium‐chain length aliphatic alcohols and aldehydes were identified as the preferred substrates. ADH3 showed ‘high‐Km’ properties with respect to ethanol, and is expected to significantly contribute to ‘first‐pass’ metabolism of alcohol. The enzyme exhibited more than two orders of magnitude higher turnover of substrate than the baboon liver ‘low‐Km’ ADH, and may play a role in the rapid metabolism of a wide range of ingested alcohols in the diet.
Original language | English (US) |
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Pages (from-to) | 922-927 |
Number of pages | 6 |
Journal | Alcoholism: Clinical and Experimental Research |
Volume | 16 |
Issue number | 5 |
DOIs | |
State | Published - Oct 1992 |
Keywords
- Alcohol Dehydrogenase
- Baboon
- Ethanol
- Stomach
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Toxicology
- Psychiatry and Mental health