A fluorescence study of type I and type II receptors of bone morphogenetic proteins with bis-ANS (4, 4′-dianilino-1, 1′-bisnaphthyl-5, 5′ disulfonic acid)

Huiran Yin, Qing Zhou, Markandeswar Panda, Lee Chuan C. Yeh, Michelle C. Zavala, John C. Lee

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Crystallography studies on several members of the bone morphogenetic protein (BMP) receptors suggested that hydrophobic regions in these proteins play an important role in their structure and function. In the present study, the environment sensitive fluorescent probe 4, 4′-dianilino-1, 1′-bisnaphthyl-5, 5′ disulfonic acid (bis-ANS) was used to study the hydrophobic regions of the extracellular domain of the type I and II receptors for bone morphogenetic proteins (ecBMPR-IB and ecBMPR-II). A single bis-ANS binding site per receptor molecule was found for both receptors, but the two receptors interacted with bis-ANS with distinctive characteristics. A significant shift in the emission maximum from 498 to 510 nm was detected when bis-ANS binds ecBMPR-IB, but a negligible change in the emission maximum was observed when the dye binds ecBMPR-II. Under identical reaction conditions, the maximum fluorescence intensities of the probe (Imax) for the ecBMPR-IB and -II are 4.0 and 6.2 × 104 arbitrary units, respectively. The probe binds to ecBMPR-IB and -II with Kd = 11.0 and 17.5 μM, respectively. The bis-ANS modified site on both receptor types was not readily accessible to acrylamide quenching. Fluorescence energy transfer experiments further revealed close proximity between the tyrosine (in ecBMPR-IB) and the tryptophan residue (in ecBMPR-II) and the respective bis-ANS binding site in these receptors. The binding of bis-ANS did not alter the ligand binding activity of ecBMPR-IB, but enhanced that of ecBMPR-II. These results show that the bis-ANS-modified hydrophobic site on the ecBMPR-IB and -II molecules plays a different functional role.

Original languageEnglish (US)
Pages (from-to)493-501
Number of pages9
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1774
Issue number4
DOIs
StatePublished - Apr 2007

Keywords

  • BMP receptors
  • Fluorescence
  • Hydrophobic surface
  • Protein structure
  • bis-ANS

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology

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