A distal activation domain is critical in the regulation of the rat androgen receptor gene promoter

C. S. Song, S. Her, M. Slomczynska, S. J. Choi, M. H. Jung, A. K. Roy, B. Chatterjee

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The far upstream region of the rat androgen receptor (AR) gene has been cloned, and the nucleotide sequence up to 2656 bp established. Nested deletion mutants of rat AR 5' flanking sequences were ligated to the luciferase reporter gene, and their promoter activities were examined in transfected COS1 cells. Results show a critical cis-acting domain located between positions -960 and -940. Deletion of this cis element resulted in a greater than 90% decrease in the promoter activity. A nuclear protein that specifically binds to this 21-nucleotide sequence was identified by gel mobility shift analysis. The -960/-940 cis element has no identity to the binding sequence of any known transcription factor. Furthermore, the cognate binding protein is present in both rat and human (HeLa) cell nuclear extracts. We conclude that a novel trans-activator interacting at the -960/-940 region plays a critical role in the regulation of AR gene expression.

Original languageEnglish (US)
Pages (from-to)779-784
Number of pages6
JournalBiochemical Journal
Volume294
Issue number3
DOIs
StatePublished - 1993

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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