A continuous alveolar macrophage cell line: Comparisons with freshly derived alveolar macrophages

Ronald J. Helmke, Victor F. German, John A. Mangos

Research output: Contribution to journalArticlepeer-review

77 Scopus citations

Abstract

Responses of a recently developed rat alveolar macrophage cell (NR8383.1) line were compared to those of freshly derived alveolar macrophages in vitro. Marked inter- and intraspecies heterogeneity in levels of phagocytosis of unopsonized Pseudomonas aeruginosa or zymosan was noted among freshly derived alveolar macrophages from rats, rabbits, and baboons. In contrast, phagocytic responses of alveolar macrophage cell line were predictable and highly reproducible. Similar results were obtained in measuring oxidative burst, as indicated by the production of H2O2 and luminol-enhanced chemiluminescence. Responses were again highly variable in freshly derived alveolar macrophages stimulated with zymosan or phorbol myristic acetate; moreover, freshly derived alveolar macrophages exhibited a wide range of chemiluminescence activity in unstimulated cultures. Results strongly suggest that data derived from the continuous alveolar macrophage culture NR8383.1 can be extrapolated to freshly derived alveolar macrophages of various species, and in many experiments will be useful in avoiding the significant animal-to-animal variance observed among freshly derived cell preparations.

Original languageEnglish (US)
Pages (from-to)44-48
Number of pages5
JournalIn Vitro Cellular & Developmental Biology
Volume25
Issue number1
DOIs
StatePublished - Jan 1989

Keywords

  • alveolar
  • in vitro
  • macrophage
  • oxidative burst
  • phagocytosis

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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