A continuous alveolar macrophage cell line: Comparisons with freshly derived alveolar macrophages

Ronald J. Helmke, Victor F. German, John A. Mangos

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Responses of a recently developed rat alveolar macrophage cell (NR8383.1) line were compared to those of freshly derived alveolar macrophages in vitro. Marked inter- and intraspecies heterogeneity in levels of phagocytosis of unopsonized Pseudomonas aeruginosa or zymosan was noted among freshly derived alveolar macrophages from rats, rabbits, and baboons. In contrast, phagocytic responses of alveolar macrophage cell line were predictable and highly reproducible. Similar results were obtained in measuring oxidative burst, as indicated by the production of H2O2 and luminol-enhanced chemiluminescence. Responses were again highly variable in freshly derived alveolar macrophages stimulated with zymosan or phorbol myristic acetate; moreover, freshly derived alveolar macrophages exhibited a wide range of chemiluminescence activity in unstimulated cultures. Results strongly suggest that data derived from the continuous alveolar macrophage culture NR8383.1 can be extrapolated to freshly derived alveolar macrophages of various species, and in many experiments will be useful in avoiding the significant animal-to-animal variance observed among freshly derived cell preparations.

Original languageEnglish (US)
Pages (from-to)44-48
Number of pages5
JournalIn Vitro Cellular & Developmental Biology
Volume25
Issue number1
DOIs
StatePublished - Jan 1989

Fingerprint

Alveolar Epithelial Cells
Alveolar Macrophages
Cells
Cell Line
Zymosan
Chemiluminescence
Luminescence
Rats
Animals
Luminol
Respiratory Burst
Papio
Phagocytosis
Pseudomonas aeruginosa
Acetates
Rabbits

Keywords

  • alveolar
  • in vitro
  • macrophage
  • oxidative burst
  • phagocytosis

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Clinical Biochemistry

Cite this

A continuous alveolar macrophage cell line : Comparisons with freshly derived alveolar macrophages. / Helmke, Ronald J.; German, Victor F.; Mangos, John A.

In: In Vitro Cellular & Developmental Biology, Vol. 25, No. 1, 01.1989, p. 44-48.

Research output: Contribution to journalArticle

Helmke, Ronald J. ; German, Victor F. ; Mangos, John A. / A continuous alveolar macrophage cell line : Comparisons with freshly derived alveolar macrophages. In: In Vitro Cellular & Developmental Biology. 1989 ; Vol. 25, No. 1. pp. 44-48.
@article{a3a19b764d54402fb4ede0eb51a5b2b6,
title = "A continuous alveolar macrophage cell line: Comparisons with freshly derived alveolar macrophages",
abstract = "Responses of a recently developed rat alveolar macrophage cell (NR8383.1) line were compared to those of freshly derived alveolar macrophages in vitro. Marked inter- and intraspecies heterogeneity in levels of phagocytosis of unopsonized Pseudomonas aeruginosa or zymosan was noted among freshly derived alveolar macrophages from rats, rabbits, and baboons. In contrast, phagocytic responses of alveolar macrophage cell line were predictable and highly reproducible. Similar results were obtained in measuring oxidative burst, as indicated by the production of H2O2 and luminol-enhanced chemiluminescence. Responses were again highly variable in freshly derived alveolar macrophages stimulated with zymosan or phorbol myristic acetate; moreover, freshly derived alveolar macrophages exhibited a wide range of chemiluminescence activity in unstimulated cultures. Results strongly suggest that data derived from the continuous alveolar macrophage culture NR8383.1 can be extrapolated to freshly derived alveolar macrophages of various species, and in many experiments will be useful in avoiding the significant animal-to-animal variance observed among freshly derived cell preparations.",
keywords = "alveolar, in vitro, macrophage, oxidative burst, phagocytosis",
author = "Helmke, {Ronald J.} and German, {Victor F.} and Mangos, {John A.}",
year = "1989",
month = "1",
doi = "10.1007/BF02624409",
language = "English (US)",
volume = "25",
pages = "44--48",
journal = "In Vitro Cellular and Developmental Biology - Plant",
issn = "1054-5476",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - A continuous alveolar macrophage cell line

T2 - Comparisons with freshly derived alveolar macrophages

AU - Helmke, Ronald J.

AU - German, Victor F.

AU - Mangos, John A.

PY - 1989/1

Y1 - 1989/1

N2 - Responses of a recently developed rat alveolar macrophage cell (NR8383.1) line were compared to those of freshly derived alveolar macrophages in vitro. Marked inter- and intraspecies heterogeneity in levels of phagocytosis of unopsonized Pseudomonas aeruginosa or zymosan was noted among freshly derived alveolar macrophages from rats, rabbits, and baboons. In contrast, phagocytic responses of alveolar macrophage cell line were predictable and highly reproducible. Similar results were obtained in measuring oxidative burst, as indicated by the production of H2O2 and luminol-enhanced chemiluminescence. Responses were again highly variable in freshly derived alveolar macrophages stimulated with zymosan or phorbol myristic acetate; moreover, freshly derived alveolar macrophages exhibited a wide range of chemiluminescence activity in unstimulated cultures. Results strongly suggest that data derived from the continuous alveolar macrophage culture NR8383.1 can be extrapolated to freshly derived alveolar macrophages of various species, and in many experiments will be useful in avoiding the significant animal-to-animal variance observed among freshly derived cell preparations.

AB - Responses of a recently developed rat alveolar macrophage cell (NR8383.1) line were compared to those of freshly derived alveolar macrophages in vitro. Marked inter- and intraspecies heterogeneity in levels of phagocytosis of unopsonized Pseudomonas aeruginosa or zymosan was noted among freshly derived alveolar macrophages from rats, rabbits, and baboons. In contrast, phagocytic responses of alveolar macrophage cell line were predictable and highly reproducible. Similar results were obtained in measuring oxidative burst, as indicated by the production of H2O2 and luminol-enhanced chemiluminescence. Responses were again highly variable in freshly derived alveolar macrophages stimulated with zymosan or phorbol myristic acetate; moreover, freshly derived alveolar macrophages exhibited a wide range of chemiluminescence activity in unstimulated cultures. Results strongly suggest that data derived from the continuous alveolar macrophage culture NR8383.1 can be extrapolated to freshly derived alveolar macrophages of various species, and in many experiments will be useful in avoiding the significant animal-to-animal variance observed among freshly derived cell preparations.

KW - alveolar

KW - in vitro

KW - macrophage

KW - oxidative burst

KW - phagocytosis

UR - http://www.scopus.com/inward/record.url?scp=0024598765&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024598765&partnerID=8YFLogxK

U2 - 10.1007/BF02624409

DO - 10.1007/BF02624409

M3 - Article

C2 - 2914814

AN - SCOPUS:0024598765

VL - 25

SP - 44

EP - 48

JO - In Vitro Cellular and Developmental Biology - Plant

JF - In Vitro Cellular and Developmental Biology - Plant

SN - 1054-5476

IS - 1

ER -