A conserved role for the 20S proteasome and Nrf2 transcription factor in oxidative stress adaptation in mammals, Caenorhabditis elegans and Drosophila melanogaster

Andrew M. Pickering, Trisha A. Staab, John Tower, Derek Sieburth, Kelvin J.A. Davies

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

In mammalian cells, hydrogen peroxide (H2O2)-induced adaptation to oxidative stress is strongly dependent on an Nrf2 transcription factor-mediated increase in the 20S proteasome. Here, we report that both Caenorhabditis elegans nematode worms and Drosophila melanogaster fruit flies are also capable of adapting to oxidative stress with H2O2 pre-treatment. As in mammalian cells, this adaptive response in worms and flies involves an increase in proteolytic activity and increased expression of the 20S proteasome, but not of the 26S proteasome. We also found that the increase in 20S proteasome expression in both worms and flies, as in mammalian cells, is important for the adaptive response, and that it is mediated by the SKN-1 and CNC-C orthologs of the mammalian Nrf2 transcription factor, respectively. These studies demonstrate that stress mechanisms operative in cell culture also apply in disparate intact organisms across a wide biological diversity.

Original languageEnglish (US)
Pages (from-to)543-553
Number of pages11
JournalJournal of Experimental Biology
Volume216
Issue number4
DOIs
StatePublished - Feb 2013

Keywords

  • 20S Proteasome
  • Caenorhabditis elegans
  • Drosophila melanogaster
  • Nrf2
  • Oxidative stress adapation
  • Proteolysis

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Molecular Biology
  • Insect Science

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