A comparison of protein synthesis by liver parenchymal cells isolated from Fischer F344 rats of various ages

John J. Coniglio, Daniel S H Liu, Arlan Richardson

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Rates of protein synthesis by intact liver parenchymal cells isolated from male Fischer F344 rats ranging in age from 2.5 to 30 months were determined by measuring the incorporation of [3H]valine into acid-insoluble material and the specific activity of the extracellular valine. The rate of protein synthesis decreased 44% from 2.5 to 18 months and then increased slightly (18%) from 18 to 30 months. There was no dramatic change in the types of proteins synthesized by isolated liver parenchymal cells isolated from 2- or 18-month-old rats as determined by SDS-polyacrylamide gel electrophoresis. The ribosomal-transit time by liver parenchymal cells isolated from 18-month-old rats was 60% higher than the ribosomal-transit time of liver parenchymal cells isolated from 4-month-old rats. The fidelity of protein synthesis by parenchymal cells isolated from 4- and 18-month old rats was compared by measuring the incorporation of p-fluorophenyl-alanine (an analogue of phenylalanine) into acid-insoluble material. Although protein synthesis decreased significantly from 4 to 18 months, the fidelity of protein synthesis remained constant.

Original languageEnglish (US)
Pages (from-to)77-90
Number of pages14
JournalMechanisms of Ageing and Development
Volume11
Issue number2
DOIs
StatePublished - 1979
Externally publishedYes

Fingerprint

Inbred F344 Rats
Liver
Rats
Proteins
Valine
Acids
Electrophoresis
Phenylalanine
Alanine
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

A comparison of protein synthesis by liver parenchymal cells isolated from Fischer F344 rats of various ages. / Coniglio, John J.; Liu, Daniel S H; Richardson, Arlan.

In: Mechanisms of Ageing and Development, Vol. 11, No. 2, 1979, p. 77-90.

Research output: Contribution to journalArticle

Coniglio, John J. ; Liu, Daniel S H ; Richardson, Arlan. / A comparison of protein synthesis by liver parenchymal cells isolated from Fischer F344 rats of various ages. In: Mechanisms of Ageing and Development. 1979 ; Vol. 11, No. 2. pp. 77-90.
@article{0e895b14ee5b499f8bd7a4113ddf4670,
title = "A comparison of protein synthesis by liver parenchymal cells isolated from Fischer F344 rats of various ages",
abstract = "Rates of protein synthesis by intact liver parenchymal cells isolated from male Fischer F344 rats ranging in age from 2.5 to 30 months were determined by measuring the incorporation of [3H]valine into acid-insoluble material and the specific activity of the extracellular valine. The rate of protein synthesis decreased 44{\%} from 2.5 to 18 months and then increased slightly (18{\%}) from 18 to 30 months. There was no dramatic change in the types of proteins synthesized by isolated liver parenchymal cells isolated from 2- or 18-month-old rats as determined by SDS-polyacrylamide gel electrophoresis. The ribosomal-transit time by liver parenchymal cells isolated from 18-month-old rats was 60{\%} higher than the ribosomal-transit time of liver parenchymal cells isolated from 4-month-old rats. The fidelity of protein synthesis by parenchymal cells isolated from 4- and 18-month old rats was compared by measuring the incorporation of p-fluorophenyl-alanine (an analogue of phenylalanine) into acid-insoluble material. Although protein synthesis decreased significantly from 4 to 18 months, the fidelity of protein synthesis remained constant.",
author = "Coniglio, {John J.} and Liu, {Daniel S H} and Arlan Richardson",
year = "1979",
doi = "10.1016/0047-6374(79)90026-5",
language = "English (US)",
volume = "11",
pages = "77--90",
journal = "Mechanisms of Ageing and Development",
issn = "0047-6374",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - A comparison of protein synthesis by liver parenchymal cells isolated from Fischer F344 rats of various ages

AU - Coniglio, John J.

AU - Liu, Daniel S H

AU - Richardson, Arlan

PY - 1979

Y1 - 1979

N2 - Rates of protein synthesis by intact liver parenchymal cells isolated from male Fischer F344 rats ranging in age from 2.5 to 30 months were determined by measuring the incorporation of [3H]valine into acid-insoluble material and the specific activity of the extracellular valine. The rate of protein synthesis decreased 44% from 2.5 to 18 months and then increased slightly (18%) from 18 to 30 months. There was no dramatic change in the types of proteins synthesized by isolated liver parenchymal cells isolated from 2- or 18-month-old rats as determined by SDS-polyacrylamide gel electrophoresis. The ribosomal-transit time by liver parenchymal cells isolated from 18-month-old rats was 60% higher than the ribosomal-transit time of liver parenchymal cells isolated from 4-month-old rats. The fidelity of protein synthesis by parenchymal cells isolated from 4- and 18-month old rats was compared by measuring the incorporation of p-fluorophenyl-alanine (an analogue of phenylalanine) into acid-insoluble material. Although protein synthesis decreased significantly from 4 to 18 months, the fidelity of protein synthesis remained constant.

AB - Rates of protein synthesis by intact liver parenchymal cells isolated from male Fischer F344 rats ranging in age from 2.5 to 30 months were determined by measuring the incorporation of [3H]valine into acid-insoluble material and the specific activity of the extracellular valine. The rate of protein synthesis decreased 44% from 2.5 to 18 months and then increased slightly (18%) from 18 to 30 months. There was no dramatic change in the types of proteins synthesized by isolated liver parenchymal cells isolated from 2- or 18-month-old rats as determined by SDS-polyacrylamide gel electrophoresis. The ribosomal-transit time by liver parenchymal cells isolated from 18-month-old rats was 60% higher than the ribosomal-transit time of liver parenchymal cells isolated from 4-month-old rats. The fidelity of protein synthesis by parenchymal cells isolated from 4- and 18-month old rats was compared by measuring the incorporation of p-fluorophenyl-alanine (an analogue of phenylalanine) into acid-insoluble material. Although protein synthesis decreased significantly from 4 to 18 months, the fidelity of protein synthesis remained constant.

UR - http://www.scopus.com/inward/record.url?scp=0018581846&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018581846&partnerID=8YFLogxK

U2 - 10.1016/0047-6374(79)90026-5

DO - 10.1016/0047-6374(79)90026-5

M3 - Article

C2 - 491778

AN - SCOPUS:0018581846

VL - 11

SP - 77

EP - 90

JO - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

SN - 0047-6374

IS - 2

ER -