Abstract
Bone remodeling is a process consisting of bone formation and resorption. The present study compared the relative osteoclastic and osteoblastic potency of bone morphogenetic proteins (BMP)-2, -4, -5, -6, and -7 in primary murine bone marrow cultures. All five BMPs stimulated, to varying degree, formation of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells in a time- and protein concentration-dependent manner. The TRAP staining intensity correlated positively with the number of nuclei per TRAP-positive cell and the mRNA levels of colony-stimulating factor (CSF-1), receptor activator of nuclear factor κB ligand (RANKL), TRAP, and cathepsin K. Under osteogenic conditions, all five BMPs stimulated AP activity and mineralized bone nodule formation in a protein concentration-dependent manner in the same primary murine bone marrow cell culture system. These findings should be useful in designing treatment strategies for bone regeneration.
Original language | English (US) |
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Pages (from-to) | 121-131 |
Number of pages | 11 |
Journal | Growth Factors |
Volume | 27 |
Issue number | 2 |
DOIs | |
State | Published - 2009 |
Externally published | Yes |
Keywords
- Bone marrow culture
- Bone morphogenetic proteins
- Gene expression
- Osteoblast differentiation
- Osteoclast differentiation
ASJC Scopus subject areas
- Endocrinology
- Clinical Biochemistry
- Cell Biology