A comparative study of neuronal and inducible nitric oxide synthases: Generation of nitric oxide, superoxide, and hydrogen peroxide

John Weaver, Supatra Porasuphatana, Pei Tsai, Sovitj Pou, Linda J. Roman, Gerald M. Rosen

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Nitric oxide synthases (NOS) independent of the isozyme, produce nitric oxide (.NO), superoxide (O2.-), and hydrogen peroxide (H2O2). Since .NO has been implicated in many physiological processes, the importance of O2.- and H2O2 in regulating cell signaling by .NO cannot be overlooked. Before addressing these questions, we investigated the production of .NO, O2.-, and H 2O2 by purified NOS. NOS 1 and NOS 2 were chosen, as the flux of .NO from each isozyme supports differential biological activity. We found that the initial rate and sustained production of .NO was considerably greater for NOS 2 as compared to NOS 1. In the absence of l-arginine, however, NOS 1 generation of O2.- and H2O2 was found to be substantially greater than that measured for NOS 2. Differences between NOS 1 and NOS 2 production of .NO, O2.-, and H2O2 may define the specific physiologic function of each isozyme.

Original languageEnglish (US)
Pages (from-to)302-308
Number of pages7
JournalBiochimica et Biophysica Acta - General Subjects
Volume1726
Issue number3
DOIs
StatePublished - Nov 30 2005

Keywords

  • Cell signaling
  • Hydrogen peroxide
  • Inducible nitric oxide synthase
  • Neuronal nitric oxide synthase
  • Nitric oxide
  • Superoxide

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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