TY - JOUR
T1 - A comparative study of neuronal and inducible nitric oxide synthases
T2 - Generation of nitric oxide, superoxide, and hydrogen peroxide
AU - Weaver, John
AU - Porasuphatana, Supatra
AU - Tsai, Pei
AU - Pou, Sovitj
AU - Roman, Linda J.
AU - Rosen, Gerald M.
N1 - Funding Information:
This research was supported in part by grants from the National Institutes of Health, EB- 2034 (GMR, JW, and SP), AG-20445 (PT), and GM-52419 (LJR) and the Robert A. Welch Foundation grant AQ1192 (LJR).
PY - 2005/11/30
Y1 - 2005/11/30
N2 - Nitric oxide synthases (NOS) independent of the isozyme, produce nitric oxide (.NO), superoxide (O2.-), and hydrogen peroxide (H2O2). Since .NO has been implicated in many physiological processes, the importance of O2.- and H2O2 in regulating cell signaling by .NO cannot be overlooked. Before addressing these questions, we investigated the production of .NO, O2.-, and H 2O2 by purified NOS. NOS 1 and NOS 2 were chosen, as the flux of .NO from each isozyme supports differential biological activity. We found that the initial rate and sustained production of .NO was considerably greater for NOS 2 as compared to NOS 1. In the absence of l-arginine, however, NOS 1 generation of O2.- and H2O2 was found to be substantially greater than that measured for NOS 2. Differences between NOS 1 and NOS 2 production of .NO, O2.-, and H2O2 may define the specific physiologic function of each isozyme.
AB - Nitric oxide synthases (NOS) independent of the isozyme, produce nitric oxide (.NO), superoxide (O2.-), and hydrogen peroxide (H2O2). Since .NO has been implicated in many physiological processes, the importance of O2.- and H2O2 in regulating cell signaling by .NO cannot be overlooked. Before addressing these questions, we investigated the production of .NO, O2.-, and H 2O2 by purified NOS. NOS 1 and NOS 2 were chosen, as the flux of .NO from each isozyme supports differential biological activity. We found that the initial rate and sustained production of .NO was considerably greater for NOS 2 as compared to NOS 1. In the absence of l-arginine, however, NOS 1 generation of O2.- and H2O2 was found to be substantially greater than that measured for NOS 2. Differences between NOS 1 and NOS 2 production of .NO, O2.-, and H2O2 may define the specific physiologic function of each isozyme.
KW - Cell signaling
KW - Hydrogen peroxide
KW - Inducible nitric oxide synthase
KW - Neuronal nitric oxide synthase
KW - Nitric oxide
KW - Superoxide
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U2 - 10.1016/j.bbagen.2005.08.012
DO - 10.1016/j.bbagen.2005.08.012
M3 - Article
C2 - 16216417
AN - SCOPUS:27944459284
VL - 1726
SP - 302
EP - 308
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0304-4165
IS - 3
ER -