A common idiotype expressed on a murine anti-Sm monoclonal antibody and antibodies in SLE sera

M. Takei, H. Dang, N. Talal

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Abstract

A rabbit anti-idiotypic antiserum made against a murine monoclonal anti-Sm autoantibody (Y2) was used in a solid-phase radioimmunoassay to investigate idiotypic cross reactivity among anti-Sm antibodies present in sera from patients with systemic lupus erythematosus. Sera from 25 of 51 SLE patients (49%) containing anti-Sm antibodies were positive for this Y2 idiotype compared to only one of 22 normal human sera. Nine of 28 SLE patients (32%) whose sera were anti-Sm negative were also positive for the Y2 idiotype in low titre. Binding was not due to rheumatoid factor-like activity but was specific for the Y2 determinant and could be eliminated by absorption with Y2 monoclonal antibodies. The anti-idiotypic antibody blocked the ability of 12 of 25 anti-Sm positive lupus sera to bind Sm. Conversely, Sm antigen inhibited the binding of anti-idiotypic antibody in nine of 12 lupus sera.

Original languageEnglish (US)
Pages (from-to)546-554
Number of pages9
JournalClinical and Experimental Immunology
Volume70
Issue number3
StatePublished - Dec 1 1987

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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