Abstract
A new enzyme immunoassay method (EMIT(TM), Syva Co.) was compared with conventional high-performance liquid chromatography (HPLC) and agar-diffusion bioassay methods for measurement of chloramphenicol in human serum. Forty-nine serum samples were assayed by each of the three methods. Excellent correlation was observed between values by EMIT and by the two conventional methods (r = 0.986 and 0.961). Precision was acceptable (CV < 5%) with EMIT. Assay of samples containing chloramphenicol glucuronide and chloramphenicol succinate demonstrated that EMIT recognizes only the biologically active (base) form of the drug. The capability to test serum samples as small as 0.2 mL, adaptation to widely available instrumentation, and provision of rapid results are principal advantages of the EMIT method for routine chloramphenicol measurements.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1872-1875 |
| Number of pages | 4 |
| Journal | Clinical Chemistry |
| Volume | 34 |
| Issue number | 9 |
| State | Published - 1988 |
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ASJC Scopus subject areas
- Clinical Biochemistry
Cite this
A commercial enzyme immunoassay method (EMIT(TM)) compared with liquid chromatography and bioassay methods for measurement of chloramphenicol. / Schwartz, J. G.; Casto, D. T.; Ayo, S.; Carnahan, J. J.; Jorgensen, J. H.
In: Clinical Chemistry, Vol. 34, No. 9, 1988, p. 1872-1875.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A commercial enzyme immunoassay method (EMIT(TM)) compared with liquid chromatography and bioassay methods for measurement of chloramphenicol
AU - Schwartz, J. G.
AU - Casto, D. T.
AU - Ayo, S.
AU - Carnahan, J. J.
AU - Jorgensen, J. H.
PY - 1988
Y1 - 1988
N2 - A new enzyme immunoassay method (EMIT(TM), Syva Co.) was compared with conventional high-performance liquid chromatography (HPLC) and agar-diffusion bioassay methods for measurement of chloramphenicol in human serum. Forty-nine serum samples were assayed by each of the three methods. Excellent correlation was observed between values by EMIT and by the two conventional methods (r = 0.986 and 0.961). Precision was acceptable (CV < 5%) with EMIT. Assay of samples containing chloramphenicol glucuronide and chloramphenicol succinate demonstrated that EMIT recognizes only the biologically active (base) form of the drug. The capability to test serum samples as small as 0.2 mL, adaptation to widely available instrumentation, and provision of rapid results are principal advantages of the EMIT method for routine chloramphenicol measurements.
AB - A new enzyme immunoassay method (EMIT(TM), Syva Co.) was compared with conventional high-performance liquid chromatography (HPLC) and agar-diffusion bioassay methods for measurement of chloramphenicol in human serum. Forty-nine serum samples were assayed by each of the three methods. Excellent correlation was observed between values by EMIT and by the two conventional methods (r = 0.986 and 0.961). Precision was acceptable (CV < 5%) with EMIT. Assay of samples containing chloramphenicol glucuronide and chloramphenicol succinate demonstrated that EMIT recognizes only the biologically active (base) form of the drug. The capability to test serum samples as small as 0.2 mL, adaptation to widely available instrumentation, and provision of rapid results are principal advantages of the EMIT method for routine chloramphenicol measurements.
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M3 - Article
C2 - 3046781
AN - SCOPUS:0023749382
VL - 34
SP - 1872
EP - 1875
JO - Clinical Chemistry
JF - Clinical Chemistry
SN - 0009-9147
IS - 9
ER -