A commercial enzyme immunoassay method (EMIT(TM)) compared with liquid chromatography and bioassay methods for measurement of chloramphenicol

J. G. Schwartz; D. T. Casto; S. Ayo; J. J. Carnahan; J. H. Jorgensen

doi:10.1093/clinchem/34.9.1870

A commercial enzyme immunoassay method (EMIT(TM)) compared with liquid chromatography and bioassay methods for measurement of chloramphenicol

J. G. Schwartz, D. T. Casto, S. Ayo, J. J. Carnahan, J. H. Jorgensen

Pathology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A new enzyme immunoassay method (EMIT(TM), Syva Co.) was compared with conventional high-performance liquid chromatography (HPLC) and agar-diffusion bioassay methods for measurement of chloramphenicol in human serum. Forty-nine serum samples were assayed by each of the three methods. Excellent correlation was observed between values by EMIT and by the two conventional methods (r = 0.986 and 0.961). Precision was acceptable (CV < 5%) with EMIT. Assay of samples containing chloramphenicol glucuronide and chloramphenicol succinate demonstrated that EMIT recognizes only the biologically active (base) form of the drug. The capability to test serum samples as small as 0.2 mL, adaptation to widely available instrumentation, and provision of rapid results are principal advantages of the EMIT method for routine chloramphenicol measurements.

Original language	English (US)
Pages (from-to)	1872-1875
Number of pages	4
Journal	Clinical Chemistry
Volume	34
Issue number	9
DOIs	https://doi.org/10.1093/clinchem/34.9.1870
State	Published - 1988

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

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title = "A commercial enzyme immunoassay method (EMIT(TM)) compared with liquid chromatography and bioassay methods for measurement of chloramphenicol",

abstract = "A new enzyme immunoassay method (EMIT(TM), Syva Co.) was compared with conventional high-performance liquid chromatography (HPLC) and agar-diffusion bioassay methods for measurement of chloramphenicol in human serum. Forty-nine serum samples were assayed by each of the three methods. Excellent correlation was observed between values by EMIT and by the two conventional methods (r = 0.986 and 0.961). Precision was acceptable (CV < 5%) with EMIT. Assay of samples containing chloramphenicol glucuronide and chloramphenicol succinate demonstrated that EMIT recognizes only the biologically active (base) form of the drug. The capability to test serum samples as small as 0.2 mL, adaptation to widely available instrumentation, and provision of rapid results are principal advantages of the EMIT method for routine chloramphenicol measurements.",

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AU - Schwartz, J. G.

AU - Casto, D. T.

AU - Ayo, S.

AU - Carnahan, J. J.

AU - Jorgensen, J. H.

PY - 1988

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