A collection of four integration-free iPSC lines derived from diagnosed sporadic Alzheimer's disease patients with different APOE alleles

Eva Díaz-Guerra; Elena P. Moreno-Jiménez; Itziar de Rojas; César Rodríguez; Eva Rodríguez-Traver; Esther Arribas-González; María Orera; Isabel Hernández; Agustín Ruiz; Carlos Vicario

doi:10.1016/j.scr.2019.101522

A collection of four integration-free iPSC lines derived from diagnosed sporadic Alzheimer's disease patients with different APOE alleles

Eva Díaz-Guerra, Elena P. Moreno-Jiménez, Itziar de Rojas, César Rodríguez, Eva Rodríguez-Traver, Esther Arribas-González, María Orera, Isabel Hernández, Agustín Ruiz, Carlos Vicario

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Abstract

Genetic polymorphism of apolipoprotein E (APOE) confers differential susceptibility to late-onset Alzheimer's disease (LOAD). The ε3 allele of APOE, the most common isoform, does not represent a risk factor for LOAD. In contrast, the ε4 allele is the strongest genetic risk factor for this disease. Here, we present the characterization of four iPSC lines generated from dermal fibroblasts of diagnosed sporadic AD patients using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could be differentiated into the three germ layers, maintained the original genotypes, and were free from Sendai vectors and reprogramming factors.

Original language	English (US)
Article number	101522
Journal	Stem Cell Research
Volume	39
DOIs	https://doi.org/10.1016/j.scr.2019.101522
State	Published - Aug 2019
Externally published	Yes

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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Díaz-Guerra, E., Moreno-Jiménez, E. P., de Rojas, I., Rodríguez, C., Rodríguez-Traver, E., Arribas-González, E., Orera, M., Hernández, I., Ruiz, A., & Vicario, C. (2019). A collection of four integration-free iPSC lines derived from diagnosed sporadic Alzheimer's disease patients with different APOE alleles. Stem Cell Research, 39, Article 101522. https://doi.org/10.1016/j.scr.2019.101522

Díaz-Guerra, E, Moreno-Jiménez, EP, de Rojas, I, Rodríguez, C, Rodríguez-Traver, E, Arribas-González, E, Orera, M, Hernández, I, Ruiz, A & Vicario, C 2019, 'A collection of four integration-free iPSC lines derived from diagnosed sporadic Alzheimer's disease patients with different APOE alleles', Stem Cell Research, vol. 39, 101522. https://doi.org/10.1016/j.scr.2019.101522

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abstract = "Genetic polymorphism of apolipoprotein E (APOE) confers differential susceptibility to late-onset Alzheimer's disease (LOAD). The ε3 allele of APOE, the most common isoform, does not represent a risk factor for LOAD. In contrast, the ε4 allele is the strongest genetic risk factor for this disease. Here, we present the characterization of four iPSC lines generated from dermal fibroblasts of diagnosed sporadic AD patients using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could be differentiated into the three germ layers, maintained the original genotypes, and were free from Sendai vectors and reprogramming factors.",

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doi = "10.1016/j.scr.2019.101522",

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AU - Díaz-Guerra, Eva

AU - Moreno-Jiménez, Elena P.

AU - de Rojas, Itziar

AU - Rodríguez, César

AU - Rodríguez-Traver, Eva

AU - Arribas-González, Esther

AU - Orera, María

AU - Hernández, Isabel

AU - Ruiz, Agustín

AU - Vicario, Carlos

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AB - Genetic polymorphism of apolipoprotein E (APOE) confers differential susceptibility to late-onset Alzheimer's disease (LOAD). The ε3 allele of APOE, the most common isoform, does not represent a risk factor for LOAD. In contrast, the ε4 allele is the strongest genetic risk factor for this disease. Here, we present the characterization of four iPSC lines generated from dermal fibroblasts of diagnosed sporadic AD patients using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could be differentiated into the three germ layers, maintained the original genotypes, and were free from Sendai vectors and reprogramming factors.

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A collection of four integration-free iPSC lines derived from diagnosed sporadic Alzheimer's disease patients with different APOE alleles

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