A cloned DNA probe identifies the sex of Schistosoma mansoni cercariae

Loretta D. Spotila; David M. Rekosh; Julie M. Boucher; Philip T. LoVerde

doi:10.1016/0166-6851(87)90125-3

A cloned DNA probe identifies the sex of Schistosoma mansoni cercariae

Loretta D. Spotila, David M. Rekosh, Julie M. Boucher, Philip T. LoVerde

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

This report describes a method for the identification of the sex of Schistosoma mansoni cercariae using a cloned DNA probe which is female-specific. The probe is a 339 bp repeat; the sequence is presented. Cercariae from nine mono-miracidially infected snails were used in a double blind study. Mice were infected and the sex of adult worms observed. DNA was isolated from cercariae, digested with EcoRI, subjected to agarose gel electrophoresis, transferred to a matrix and hybridized with the cloned female-specific DNA probe. In all nine cases the sex of the cercariae as determined by DNA analysis agreed with the sex of the adult parasites.

Original language	English (US)
Pages (from-to)	17-20
Number of pages	4
Journal	Molecular and Biochemical Parasitology
Volume	26
Issue number	1-2
DOIs	https://doi.org/10.1016/0166-6851(87)90125-3
State	Published - Nov 1987
Externally published	Yes

Keywords

Cercarial sex
Female DNA probe
Repeated DNA
Schistosomiasis

ASJC Scopus subject areas

Molecular Biology
Parasitology

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10.1016/0166-6851(87)90125-3

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title = "A cloned DNA probe identifies the sex of Schistosoma mansoni cercariae",

abstract = "This report describes a method for the identification of the sex of Schistosoma mansoni cercariae using a cloned DNA probe which is female-specific. The probe is a 339 bp repeat; the sequence is presented. Cercariae from nine mono-miracidially infected snails were used in a double blind study. Mice were infected and the sex of adult worms observed. DNA was isolated from cercariae, digested with EcoRI, subjected to agarose gel electrophoresis, transferred to a matrix and hybridized with the cloned female-specific DNA probe. In all nine cases the sex of the cercariae as determined by DNA analysis agreed with the sex of the adult parasites.",

keywords = "Cercarial sex, Female DNA probe, Repeated DNA, Schistosomiasis",

author = "Spotila, {Loretta D.} and Rekosh, {David M.} and Boucher, {Julie M.} and LoVerde, {Philip T.}",

note = "Funding Information: The authors wish to thank Dr. T. McCutchan for providing the clone pSM388, K. Zoghby and L. Spencer for initiating the mono-miracidial infections, W. Burke for instruction in sequencing and T. Wnuk for preparation of the manuscript. This work was funded by a grant from the Na- tional Institute of Allergy and Infectious Diseases (R22AI-18867), a grant from the Edna McConnell Clark Foundation, the Research Career Development Award A000905 (D.M.R.) from the National Cancer Institute, and an Individual National Research Service Award AI07161-02 (L.D.S.). This study was conducted as part of the Center for Applied Molecular Biology and Immunology of the State University of New York at Buffalo.",

year = "1987",

month = nov,

doi = "10.1016/0166-6851(87)90125-3",

language = "English (US)",

volume = "26",

pages = "17--20",

journal = "Molecular and Biochemical Parasitology",

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AU - Spotila, Loretta D.

AU - Rekosh, David M.

AU - Boucher, Julie M.

AU - LoVerde, Philip T.

N1 - Funding Information: The authors wish to thank Dr. T. McCutchan for providing the clone pSM388, K. Zoghby and L. Spencer for initiating the mono-miracidial infections, W. Burke for instruction in sequencing and T. Wnuk for preparation of the manuscript. This work was funded by a grant from the Na- tional Institute of Allergy and Infectious Diseases (R22AI-18867), a grant from the Edna McConnell Clark Foundation, the Research Career Development Award A000905 (D.M.R.) from the National Cancer Institute, and an Individual National Research Service Award AI07161-02 (L.D.S.). This study was conducted as part of the Center for Applied Molecular Biology and Immunology of the State University of New York at Buffalo.

PY - 1987/11

Y1 - 1987/11

N2 - This report describes a method for the identification of the sex of Schistosoma mansoni cercariae using a cloned DNA probe which is female-specific. The probe is a 339 bp repeat; the sequence is presented. Cercariae from nine mono-miracidially infected snails were used in a double blind study. Mice were infected and the sex of adult worms observed. DNA was isolated from cercariae, digested with EcoRI, subjected to agarose gel electrophoresis, transferred to a matrix and hybridized with the cloned female-specific DNA probe. In all nine cases the sex of the cercariae as determined by DNA analysis agreed with the sex of the adult parasites.

AB - This report describes a method for the identification of the sex of Schistosoma mansoni cercariae using a cloned DNA probe which is female-specific. The probe is a 339 bp repeat; the sequence is presented. Cercariae from nine mono-miracidially infected snails were used in a double blind study. Mice were infected and the sex of adult worms observed. DNA was isolated from cercariae, digested with EcoRI, subjected to agarose gel electrophoresis, transferred to a matrix and hybridized with the cloned female-specific DNA probe. In all nine cases the sex of the cercariae as determined by DNA analysis agreed with the sex of the adult parasites.

KW - Cercarial sex

KW - Female DNA probe

KW - Repeated DNA

KW - Schistosomiasis

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A cloned DNA probe identifies the sex of Schistosoma mansoni cercariae

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