A cloned DNA probe identifies the sex of Schistosoma mansoni cercariae

Loretta D. Spotila, David M. Rekosh, Julie M. Boucher, Philip T. LoVerde

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29 Scopus citations

Abstract

This report describes a method for the identification of the sex of Schistosoma mansoni cercariae using a cloned DNA probe which is female-specific. The probe is a 339 bp repeat; the sequence is presented. Cercariae from nine mono-miracidially infected snails were used in a double blind study. Mice were infected and the sex of adult worms observed. DNA was isolated from cercariae, digested with EcoRI, subjected to agarose gel electrophoresis, transferred to a matrix and hybridized with the cloned female-specific DNA probe. In all nine cases the sex of the cercariae as determined by DNA analysis agreed with the sex of the adult parasites.

Original languageEnglish (US)
Pages (from-to)17-20
Number of pages4
JournalMolecular and Biochemical Parasitology
Volume26
Issue number1-2
DOIs
StatePublished - Nov 1987

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Keywords

  • Cercarial sex
  • Female DNA probe
  • Repeated DNA
  • Schistosomiasis

ASJC Scopus subject areas

  • Parasitology
  • Molecular Biology

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