A Biosensor for L-Tryptophan Determination Based on Recombinant Pseudornonas savastanoi Tryptophan-2-Monooxygenase

A. L. Simonianl; E. I. Rainina; J. Wild; P. F. Fitzpatrick

doi:10.1080/00032719508000353

A Biosensor for L-Tryptophan Determination Based on Recombinant Pseudornonas savastanoi Tryptophan-2-Monooxygenase

A. L. Simonianl, E. I. Rainina, J. Wild, P. F. Fitzpatrick

Biochemistry & Structural Biology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A simple, rapid and selective biosensor for reagent-less L-tryptophan determination has been developed. Tryptophan-2-monooxygenase was immobilized on silica gel. A Clark-type membrane 02 electrode was used as a physical transducer. A positive correlation between signal and L-tryptophan concentration was observed.

Original language	English (US)
Pages (from-to)	1751-1761
Number of pages	11
Journal	Analytical Letters
Volume	28
Issue number	10
DOIs	https://doi.org/10.1080/00032719508000353
State	Published - 1995

Keywords

L-tryptophan
L-tryptophan-2-Monooxygenase
biosensor

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Spectroscopy
Clinical Biochemistry
Biochemistry, medical
Electrochemistry

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10.1080/00032719508000353

Cite this

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title = "A Biosensor for L-Tryptophan Determination Based on Recombinant Pseudornonas savastanoi Tryptophan-2-Monooxygenase",

abstract = "A simple, rapid and selective biosensor for reagent-less L-tryptophan determination has been developed. Tryptophan-2-monooxygenase was immobilized on silica gel. A Clark-type membrane 02 electrode was used as a physical transducer. A positive correlation between signal and L-tryptophan concentration was observed.",

keywords = "L-tryptophan, L-tryptophan-2-Monooxygenase, biosensor",

author = "Simonianl, {A. L.} and Rainina, {E. I.} and J. Wild and Fitzpatrick, {P. F.}",

note = "Funding Information: study. This research was also supported in part by International Science Foundation Funding Information: This research was supported in part by the National Science Foundation",

year = "1995",

doi = "10.1080/00032719508000353",

language = "English (US)",

volume = "28",

pages = "1751--1761",

journal = "Analytical Letters",

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T1 - A Biosensor for L-Tryptophan Determination Based on Recombinant Pseudornonas savastanoi Tryptophan-2-Monooxygenase

AU - Simonianl, A. L.

AU - Rainina, E. I.

AU - Wild, J.

AU - Fitzpatrick, P. F.

N1 - Funding Information: study. This research was also supported in part by International Science Foundation Funding Information: This research was supported in part by the National Science Foundation

PY - 1995

Y1 - 1995

N2 - A simple, rapid and selective biosensor for reagent-less L-tryptophan determination has been developed. Tryptophan-2-monooxygenase was immobilized on silica gel. A Clark-type membrane 02 electrode was used as a physical transducer. A positive correlation between signal and L-tryptophan concentration was observed.

AB - A simple, rapid and selective biosensor for reagent-less L-tryptophan determination has been developed. Tryptophan-2-monooxygenase was immobilized on silica gel. A Clark-type membrane 02 electrode was used as a physical transducer. A positive correlation between signal and L-tryptophan concentration was observed.

KW - L-tryptophan

KW - L-tryptophan-2-Monooxygenase

KW - biosensor

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DO - 10.1080/00032719508000353

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EP - 1761

JO - Analytical Letters

JF - Analytical Letters

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IS - 10

ER -

A Biosensor for L-Tryptophan Determination Based on Recombinant Pseudornonas savastanoi Tryptophan-2-Monooxygenase

Abstract

Keywords

ASJC Scopus subject areas

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