The general stress regulon of Bacillus subtilis is controlled by the transcription factor σ(B). Environmental stress activates σ(B) via a phosphatase/kinase cascade that triggers σ(B)'s release from an anti sigma factor complex. To determine if the members of the phosphatase/kinase cascade are sufficient to detect environmental stress and activate σ(B), we expressed σ(B) and its regulators in E. coli. In E. coli, as in B. subtilis, the intact collection of regulators silenced σ(B), while allowing σB to be active if the cascade's most upstream negative regulator was deleted. The regulators could not, however, activate σ(B) in response to ethanol treatment or heat shock. In other experiments, the GroEL and DnaK chaperones, known to be important in controlling stress a factors in E. coli, were found to be unimportant for σ(B) activity in B. subtilis. The findings argue that stress induction of σ(B) requires novel factors that are B. subtilis specific.
|Original language||English (US)|
|Number of pages||5|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Apr 2 1999|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology