A Bacillus-specific factor is needed to trigger the stress-activated phosphatase/kinase cascade of σ(B) induction

Janelle M. Scott, Natalya Smirnova, W. G. Haldenwang

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The general stress regulon of Bacillus subtilis is controlled by the transcription factor σ(B). Environmental stress activates σ(B) via a phosphatase/kinase cascade that triggers σ(B)'s release from an anti sigma factor complex. To determine if the members of the phosphatase/kinase cascade are sufficient to detect environmental stress and activate σ(B), we expressed σ(B) and its regulators in E. coli. In E. coli, as in B. subtilis, the intact collection of regulators silenced σ(B), while allowing σB to be active if the cascade's most upstream negative regulator was deleted. The regulators could not, however, activate σ(B) in response to ethanol treatment or heat shock. In other experiments, the GroEL and DnaK chaperones, known to be important in controlling stress a factors in E. coli, were found to be unimportant for σ(B) activity in B. subtilis. The findings argue that stress induction of σ(B) requires novel factors that are B. subtilis specific.

Original languageEnglish (US)
Pages (from-to)106-110
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume257
Issue number1
DOIs
StatePublished - Apr 2 1999

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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