5-HT(1B) receptor-mediated regulation of serotonin clearance in rat hippocampus in vivo

Lynette C Daws, Georgianna G Gould, S. D. Teicher, G. A. Gerhardt, Alan Frazer

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The 5-hydroxytryptamine (5-HT; serotonin) transporter (5-HTT) is important in terminating serotonergic neurotransmission and is a primary target for many psychotherapeutic drugs. Study of the regulation of 5-HTT activity is therefore important in understanding the control of serotonergic neurotransmission. Using highspeed chronoamperometry, we have demonstrated that local application of 5-HT(1B) antagonists into the CA3 region of the hippocampus prolongs the clearance of 5-HT from extracellular fluid (ECF). In the present study, we demonstrate that the 5-HT(1B) antagonist cyanopindolol does not produce this effect by increasing release of endogenous 5-HT or by directly binding to the 5-HTT. Dose-response studies showed that the potency of cyanopindolol to inhibit clearance of 5-HT was equivalent to that of the selective 5-HT reuptake inhibitor fluvoxamine. Local application of the 5-HT(1A) antagonist WAY 100635 did not alter 5-HT clearance, suggesting that the effect of cyanopindolol to prolong clearance is not via a mechanism involving 5-HT(1A) receptors. Finally, the effect of low doses of cyanopindolol and fluvoxamine to inhibit clearance of 5-HT from ECF was additive. These data are consistent with the hypothesis that activation of terminal 5-HT(1B) autoreceptors increases 5-HTT activity.

Original languageEnglish (US)
Pages (from-to)2113-2122
Number of pages10
JournalJournal of Neurochemistry
Volume75
Issue number5
DOIs
StatePublished - 2000

Fingerprint

Receptor, Serotonin, 5-HT1B
Rats
Hippocampus
Serotonin
Serotonin Plasma Membrane Transport Proteins
Serotonin Antagonists
Fluvoxamine
Extracellular Fluid
Synaptic Transmission
Autoreceptors
Receptor, Serotonin, 5-HT1A
Chronoamperometry
Fluids

Keywords

  • -5-HT(1B) autoreceptor
  • Chronoamperometry
  • Hippocampus
  • Rat
  • Serotonin transporter

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

5-HT(1B) receptor-mediated regulation of serotonin clearance in rat hippocampus in vivo. / Daws, Lynette C; Gould, Georgianna G; Teicher, S. D.; Gerhardt, G. A.; Frazer, Alan.

In: Journal of Neurochemistry, Vol. 75, No. 5, 2000, p. 2113-2122.

Research output: Contribution to journalArticle

Daws, Lynette C ; Gould, Georgianna G ; Teicher, S. D. ; Gerhardt, G. A. ; Frazer, Alan. / 5-HT(1B) receptor-mediated regulation of serotonin clearance in rat hippocampus in vivo. In: Journal of Neurochemistry. 2000 ; Vol. 75, No. 5. pp. 2113-2122.
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