3D magnetic resonance microscopy of the ex vivo retina

Bryan H. De La Garza, Eric R. Muir, Yen Yu I Shih, Timothy Q. Duong

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

3D-MR microscopy at 11.7T and 20 × 20 × 57 μm resolution was performed on formalin-fixed rat eyes with: (I) no contrast agent and (II) Gadodiamide (Omniscan) added to the fixative. Group I data showed generally poor contrast among layers. Group II data showed markedly better lamina-specific contrast with the nerve fiber + ganglion cell layer and inner nuclear layer being hypointense, and the inner plexiform, outer plexiform, outer nuclear layer, and the segments being hyperintense. The signal-to-noise ratio in group II was higher than group I, consistent with Gadodiamide acting as a T 1-contrast agent. All major retinal layers were assigned and their thicknesses quantified with corroboration by histology. MR microscopy allows nondestructive examination of valuable specimens and could have applications in disease and in vivo.

Original languageEnglish (US)
Pages (from-to)1154-1158
Number of pages5
JournalMagnetic Resonance in Medicine
Volume67
Issue number4
DOIs
StatePublished - Apr 2012

Fingerprint

gadodiamide
Retina
Microscopy
Magnetic Resonance Spectroscopy
Contrast Media
Fixatives
Signal-To-Noise Ratio
Nerve Fibers
Ganglia
Formaldehyde
Histology

Keywords

  • high field
  • manganese
  • MEMRI
  • MRI contrast agents
  • photoreceptors
  • T and T contrast
  • ultra high-resolution MRI

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

De La Garza, B. H., Muir, E. R., Shih, Y. Y. I., & Duong, T. Q. (2012). 3D magnetic resonance microscopy of the ex vivo retina. Magnetic Resonance in Medicine, 67(4), 1154-1158. https://doi.org/10.1002/mrm.23082

3D magnetic resonance microscopy of the ex vivo retina. / De La Garza, Bryan H.; Muir, Eric R.; Shih, Yen Yu I; Duong, Timothy Q.

In: Magnetic Resonance in Medicine, Vol. 67, No. 4, 04.2012, p. 1154-1158.

Research output: Contribution to journalArticle

De La Garza, BH, Muir, ER, Shih, YYI & Duong, TQ 2012, '3D magnetic resonance microscopy of the ex vivo retina', Magnetic Resonance in Medicine, vol. 67, no. 4, pp. 1154-1158. https://doi.org/10.1002/mrm.23082
De La Garza, Bryan H. ; Muir, Eric R. ; Shih, Yen Yu I ; Duong, Timothy Q. / 3D magnetic resonance microscopy of the ex vivo retina. In: Magnetic Resonance in Medicine. 2012 ; Vol. 67, No. 4. pp. 1154-1158.
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