Abstract
The degree of lipid peroxidation (LPO) as indicated by the levels of thiobarbituric acid reactive substances, malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA), and the activity of sorbitol dehydrogenase (SDH) in serum as parameters of hepatotoxicity were studied in rats treated with a single intraperitoneal (ip) injection of the hepatocarcinogen 2-nitropropane (2-NP). Since melatonin, the main secretory product of the pineal gland, has been shown to protect against a number of toxic agents, it was given 30 min before 2-NP to test its protective effect against 2-NP toxicity. Significant increases in LPO in liver (P<0.0001), lung (P<0.05) and kidney (P<0.0001) were observed 24 h after 4 mmol/kg 2-NP while serum SDH activity was increased 470-fold. All parameters showed time (0, 4, 8, 24 h) and dose (0, 1, 2, 3, 4 mmol/kg) dependency. The induction of LPO by 2-NP was significantly reduced in lung and kidney when melatonin (2.5, 5 or 10 mg/kg) was given prior to 2-NP administration. The elevation in serum SDH caused by 2-NP was also reduced when melatonin was given. These findings show that 2-NP induces LPO and that pharmacological levels of melatonin can reduce the toxicity of this hepatocarcinogen. Copyright (C) 1998 Elsevier Science Ireland Ltd.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 183-190 |
| Number of pages | 8 |
| Journal | Toxicology |
| Volume | 130 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Sep 15 1998 |
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Keywords
- 2-Nitropropane
- Free radicals
- Lipid peroxidation
- Melatonin
- Reactive oxygen species
- Sorbitol dehydrogenase
ASJC Scopus subject areas
- Toxicology
Cite this
2-Nitropropane-induced lipid peroxidation : Antitoxic effects of melatonin. / Joong Kim, Seok; Reiter, Russel J; Garay, M. Veronica Rouvier; Qi, Wenbo; El-Sokkary, Gamal H.; Tan, Dun Xian.
In: Toxicology, Vol. 130, No. 2-3, 15.09.1998, p. 183-190.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 2-Nitropropane-induced lipid peroxidation
T2 - Antitoxic effects of melatonin
AU - Joong Kim, Seok
AU - Reiter, Russel J
AU - Garay, M. Veronica Rouvier
AU - Qi, Wenbo
AU - El-Sokkary, Gamal H.
AU - Tan, Dun Xian
PY - 1998/9/15
Y1 - 1998/9/15
N2 - The degree of lipid peroxidation (LPO) as indicated by the levels of thiobarbituric acid reactive substances, malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA), and the activity of sorbitol dehydrogenase (SDH) in serum as parameters of hepatotoxicity were studied in rats treated with a single intraperitoneal (ip) injection of the hepatocarcinogen 2-nitropropane (2-NP). Since melatonin, the main secretory product of the pineal gland, has been shown to protect against a number of toxic agents, it was given 30 min before 2-NP to test its protective effect against 2-NP toxicity. Significant increases in LPO in liver (P<0.0001), lung (P<0.05) and kidney (P<0.0001) were observed 24 h after 4 mmol/kg 2-NP while serum SDH activity was increased 470-fold. All parameters showed time (0, 4, 8, 24 h) and dose (0, 1, 2, 3, 4 mmol/kg) dependency. The induction of LPO by 2-NP was significantly reduced in lung and kidney when melatonin (2.5, 5 or 10 mg/kg) was given prior to 2-NP administration. The elevation in serum SDH caused by 2-NP was also reduced when melatonin was given. These findings show that 2-NP induces LPO and that pharmacological levels of melatonin can reduce the toxicity of this hepatocarcinogen. Copyright (C) 1998 Elsevier Science Ireland Ltd.
AB - The degree of lipid peroxidation (LPO) as indicated by the levels of thiobarbituric acid reactive substances, malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA), and the activity of sorbitol dehydrogenase (SDH) in serum as parameters of hepatotoxicity were studied in rats treated with a single intraperitoneal (ip) injection of the hepatocarcinogen 2-nitropropane (2-NP). Since melatonin, the main secretory product of the pineal gland, has been shown to protect against a number of toxic agents, it was given 30 min before 2-NP to test its protective effect against 2-NP toxicity. Significant increases in LPO in liver (P<0.0001), lung (P<0.05) and kidney (P<0.0001) were observed 24 h after 4 mmol/kg 2-NP while serum SDH activity was increased 470-fold. All parameters showed time (0, 4, 8, 24 h) and dose (0, 1, 2, 3, 4 mmol/kg) dependency. The induction of LPO by 2-NP was significantly reduced in lung and kidney when melatonin (2.5, 5 or 10 mg/kg) was given prior to 2-NP administration. The elevation in serum SDH caused by 2-NP was also reduced when melatonin was given. These findings show that 2-NP induces LPO and that pharmacological levels of melatonin can reduce the toxicity of this hepatocarcinogen. Copyright (C) 1998 Elsevier Science Ireland Ltd.
KW - 2-Nitropropane
KW - Free radicals
KW - Lipid peroxidation
KW - Melatonin
KW - Reactive oxygen species
KW - Sorbitol dehydrogenase
UR - http://www.scopus.com/inward/record.url?scp=0032531263&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032531263&partnerID=8YFLogxK
U2 - 10.1016/S0300-483X(98)00111-5
DO - 10.1016/S0300-483X(98)00111-5
M3 - Article
VL - 130
SP - 183
EP - 190
JO - Toxicology
JF - Toxicology
SN - 0300-483X
IS - 2-3
ER -