TY - JOUR
T1 - 2-Nitropropane-induced lipid peroxidation
T2 - Antitoxic effects of melatonin
AU - Joong Kim, Seok
AU - Reiter, Russel J.
AU - Garay, M. Veronica Rouvier
AU - Qi, Wenbo
AU - El-Sokkary, Gamal H.
AU - Tan, Dun Xian
N1 - Funding Information:
Seok Joong Kim thanks the Korea Science and Engineering Foundation for financial support. Gamal H. El-Sokkary was supported by Egyptian Government.
Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1998/9/15
Y1 - 1998/9/15
N2 - The degree of lipid peroxidation (LPO) as indicated by the levels of thiobarbituric acid reactive substances, malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA), and the activity of sorbitol dehydrogenase (SDH) in serum as parameters of hepatotoxicity were studied in rats treated with a single intraperitoneal (ip) injection of the hepatocarcinogen 2-nitropropane (2-NP). Since melatonin, the main secretory product of the pineal gland, has been shown to protect against a number of toxic agents, it was given 30 min before 2-NP to test its protective effect against 2-NP toxicity. Significant increases in LPO in liver (P<0.0001), lung (P<0.05) and kidney (P<0.0001) were observed 24 h after 4 mmol/kg 2-NP while serum SDH activity was increased 470-fold. All parameters showed time (0, 4, 8, 24 h) and dose (0, 1, 2, 3, 4 mmol/kg) dependency. The induction of LPO by 2-NP was significantly reduced in lung and kidney when melatonin (2.5, 5 or 10 mg/kg) was given prior to 2-NP administration. The elevation in serum SDH caused by 2-NP was also reduced when melatonin was given. These findings show that 2-NP induces LPO and that pharmacological levels of melatonin can reduce the toxicity of this hepatocarcinogen. Copyright (C) 1998 Elsevier Science Ireland Ltd.
AB - The degree of lipid peroxidation (LPO) as indicated by the levels of thiobarbituric acid reactive substances, malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA), and the activity of sorbitol dehydrogenase (SDH) in serum as parameters of hepatotoxicity were studied in rats treated with a single intraperitoneal (ip) injection of the hepatocarcinogen 2-nitropropane (2-NP). Since melatonin, the main secretory product of the pineal gland, has been shown to protect against a number of toxic agents, it was given 30 min before 2-NP to test its protective effect against 2-NP toxicity. Significant increases in LPO in liver (P<0.0001), lung (P<0.05) and kidney (P<0.0001) were observed 24 h after 4 mmol/kg 2-NP while serum SDH activity was increased 470-fold. All parameters showed time (0, 4, 8, 24 h) and dose (0, 1, 2, 3, 4 mmol/kg) dependency. The induction of LPO by 2-NP was significantly reduced in lung and kidney when melatonin (2.5, 5 or 10 mg/kg) was given prior to 2-NP administration. The elevation in serum SDH caused by 2-NP was also reduced when melatonin was given. These findings show that 2-NP induces LPO and that pharmacological levels of melatonin can reduce the toxicity of this hepatocarcinogen. Copyright (C) 1998 Elsevier Science Ireland Ltd.
KW - 2-Nitropropane
KW - Free radicals
KW - Lipid peroxidation
KW - Melatonin
KW - Reactive oxygen species
KW - Sorbitol dehydrogenase
UR - http://www.scopus.com/inward/record.url?scp=0032531263&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032531263&partnerID=8YFLogxK
U2 - 10.1016/S0300-483X(98)00111-5
DO - 10.1016/S0300-483X(98)00111-5
M3 - Article
C2 - 9865485
AN - SCOPUS:0032531263
VL - 130
SP - 183
EP - 190
JO - Toxicology
JF - Toxicology
SN - 0300-483X
IS - 2-3
ER -