2-Nitropropane-induced lipid peroxidation: Antitoxic effects of melatonin

Seok Joong Kim, Russel J Reiter, M. Veronica Rouvier Garay, Wenbo Qi, Gamal H. El-Sokkary, Dun Xian Tan

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The degree of lipid peroxidation (LPO) as indicated by the levels of thiobarbituric acid reactive substances, malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA), and the activity of sorbitol dehydrogenase (SDH) in serum as parameters of hepatotoxicity were studied in rats treated with a single intraperitoneal (ip) injection of the hepatocarcinogen 2-nitropropane (2-NP). Since melatonin, the main secretory product of the pineal gland, has been shown to protect against a number of toxic agents, it was given 30 min before 2-NP to test its protective effect against 2-NP toxicity. Significant increases in LPO in liver (P<0.0001), lung (P<0.05) and kidney (P<0.0001) were observed 24 h after 4 mmol/kg 2-NP while serum SDH activity was increased 470-fold. All parameters showed time (0, 4, 8, 24 h) and dose (0, 1, 2, 3, 4 mmol/kg) dependency. The induction of LPO by 2-NP was significantly reduced in lung and kidney when melatonin (2.5, 5 or 10 mg/kg) was given prior to 2-NP administration. The elevation in serum SDH caused by 2-NP was also reduced when melatonin was given. These findings show that 2-NP induces LPO and that pharmacological levels of melatonin can reduce the toxicity of this hepatocarcinogen. Copyright (C) 1998 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)183-190
Number of pages8
JournalToxicology
Volume130
Issue number2-3
DOIs
StatePublished - Sep 15 1998

Fingerprint

Melatonin
Lipid Peroxidation
Lipids
L-Iditol 2-Dehydrogenase
Toxicity
Serum
Kidney
Lung
Pineal Gland
Thiobarbituric Acid Reactive Substances
2-nitropropane
Poisons
Intraperitoneal Injections
Malondialdehyde
Liver
Rats
Pharmacology

Keywords

  • 2-Nitropropane
  • Free radicals
  • Lipid peroxidation
  • Melatonin
  • Reactive oxygen species
  • Sorbitol dehydrogenase

ASJC Scopus subject areas

  • Toxicology

Cite this

Joong Kim, S., Reiter, R. J., Garay, M. V. R., Qi, W., El-Sokkary, G. H., & Tan, D. X. (1998). 2-Nitropropane-induced lipid peroxidation: Antitoxic effects of melatonin. Toxicology, 130(2-3), 183-190. https://doi.org/10.1016/S0300-483X(98)00111-5

2-Nitropropane-induced lipid peroxidation : Antitoxic effects of melatonin. / Joong Kim, Seok; Reiter, Russel J; Garay, M. Veronica Rouvier; Qi, Wenbo; El-Sokkary, Gamal H.; Tan, Dun Xian.

In: Toxicology, Vol. 130, No. 2-3, 15.09.1998, p. 183-190.

Research output: Contribution to journalArticle

Joong Kim, S, Reiter, RJ, Garay, MVR, Qi, W, El-Sokkary, GH & Tan, DX 1998, '2-Nitropropane-induced lipid peroxidation: Antitoxic effects of melatonin', Toxicology, vol. 130, no. 2-3, pp. 183-190. https://doi.org/10.1016/S0300-483X(98)00111-5
Joong Kim, Seok ; Reiter, Russel J ; Garay, M. Veronica Rouvier ; Qi, Wenbo ; El-Sokkary, Gamal H. ; Tan, Dun Xian. / 2-Nitropropane-induced lipid peroxidation : Antitoxic effects of melatonin. In: Toxicology. 1998 ; Vol. 130, No. 2-3. pp. 183-190.
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