1,25-dihydroxyvitamin D maintains bone cell activity, and parathyroid hormone modulates bone cell number in dogs

H. H. Malluche, C. Matthews, M. C. Faugere, P. Fanti, D. B. Endres, R. M. Friedler

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The singular and combined effects of 1,25-dihydroxyvitamin D [1,25-(OH)2D] and PTH on bone were evaluated in a long term in vivo study in dogs. Dogs were rendered deficient in 1,25-(OH)2D and PTH by five sixths nephrectomy and parathyroidectomy. A control group was sham operated. Various combinations in status of 1,25-(OH)2D and PTH were produced by daily sc injections of 1,25-(OH)2D (1.25) and/or continuous infusion of 1-34 bovine PTH. These were 1.25+/PTH+, 1.25-/PTH-, 1.25+/PTH-, 1.25-/PTH+. Serum calcium levels were kept in the normal range by the administration of one or two of the hormones or by oral supplementation of calcium lactate. Histomorphometric evaluation of static and dynamic parameters of bone after 8 months of experimental observation revealed that deficiency in 1,25-(OH)2D and PTH resulted in decreased number and activity of bone-forming and resorbing cells. Administration of 1,25-(OH)2D increased the activity but not the number of bone cells. In contrast, administration of PTH increased the number but not the activity of bone cells. Tissue level activity was decreased when one or both hormones were deficient, and normal tissue level activity was found only when both hormones were given. These data are relevant for understanding and management of diseases with perturbations in vitamin D and/or PTH.

Original languageEnglish (US)
Pages (from-to)1298-1304
Number of pages7
JournalEndocrinology
Volume119
Issue number3
StatePublished - 1986
Externally publishedYes

Fingerprint

Parathyroid Hormone
Cell Count
Dogs
Bone and Bones
Hormones
Parathyroidectomy
Disease Management
Nephrectomy
Vitamin D
Reference Values
Observation
Calcium
Control Groups
Injections
1,25-dihydroxyvitamin D
Serum

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Malluche, H. H., Matthews, C., Faugere, M. C., Fanti, P., Endres, D. B., & Friedler, R. M. (1986). 1,25-dihydroxyvitamin D maintains bone cell activity, and parathyroid hormone modulates bone cell number in dogs. Endocrinology, 119(3), 1298-1304.

1,25-dihydroxyvitamin D maintains bone cell activity, and parathyroid hormone modulates bone cell number in dogs. / Malluche, H. H.; Matthews, C.; Faugere, M. C.; Fanti, P.; Endres, D. B.; Friedler, R. M.

In: Endocrinology, Vol. 119, No. 3, 1986, p. 1298-1304.

Research output: Contribution to journalArticle

Malluche, HH, Matthews, C, Faugere, MC, Fanti, P, Endres, DB & Friedler, RM 1986, '1,25-dihydroxyvitamin D maintains bone cell activity, and parathyroid hormone modulates bone cell number in dogs', Endocrinology, vol. 119, no. 3, pp. 1298-1304.
Malluche HH, Matthews C, Faugere MC, Fanti P, Endres DB, Friedler RM. 1,25-dihydroxyvitamin D maintains bone cell activity, and parathyroid hormone modulates bone cell number in dogs. Endocrinology. 1986;119(3):1298-1304.
Malluche, H. H. ; Matthews, C. ; Faugere, M. C. ; Fanti, P. ; Endres, D. B. ; Friedler, R. M. / 1,25-dihydroxyvitamin D maintains bone cell activity, and parathyroid hormone modulates bone cell number in dogs. In: Endocrinology. 1986 ; Vol. 119, No. 3. pp. 1298-1304.
@article{aae00d729c344477bdc044df0808e300,
title = "1,25-dihydroxyvitamin D maintains bone cell activity, and parathyroid hormone modulates bone cell number in dogs",
abstract = "The singular and combined effects of 1,25-dihydroxyvitamin D [1,25-(OH)2D] and PTH on bone were evaluated in a long term in vivo study in dogs. Dogs were rendered deficient in 1,25-(OH)2D and PTH by five sixths nephrectomy and parathyroidectomy. A control group was sham operated. Various combinations in status of 1,25-(OH)2D and PTH were produced by daily sc injections of 1,25-(OH)2D (1.25) and/or continuous infusion of 1-34 bovine PTH. These were 1.25+/PTH+, 1.25-/PTH-, 1.25+/PTH-, 1.25-/PTH+. Serum calcium levels were kept in the normal range by the administration of one or two of the hormones or by oral supplementation of calcium lactate. Histomorphometric evaluation of static and dynamic parameters of bone after 8 months of experimental observation revealed that deficiency in 1,25-(OH)2D and PTH resulted in decreased number and activity of bone-forming and resorbing cells. Administration of 1,25-(OH)2D increased the activity but not the number of bone cells. In contrast, administration of PTH increased the number but not the activity of bone cells. Tissue level activity was decreased when one or both hormones were deficient, and normal tissue level activity was found only when both hormones were given. These data are relevant for understanding and management of diseases with perturbations in vitamin D and/or PTH.",
author = "Malluche, {H. H.} and C. Matthews and Faugere, {M. C.} and P. Fanti and Endres, {D. B.} and Friedler, {R. M.}",
year = "1986",
language = "English (US)",
volume = "119",
pages = "1298--1304",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "3",

}

TY - JOUR

T1 - 1,25-dihydroxyvitamin D maintains bone cell activity, and parathyroid hormone modulates bone cell number in dogs

AU - Malluche, H. H.

AU - Matthews, C.

AU - Faugere, M. C.

AU - Fanti, P.

AU - Endres, D. B.

AU - Friedler, R. M.

PY - 1986

Y1 - 1986

N2 - The singular and combined effects of 1,25-dihydroxyvitamin D [1,25-(OH)2D] and PTH on bone were evaluated in a long term in vivo study in dogs. Dogs were rendered deficient in 1,25-(OH)2D and PTH by five sixths nephrectomy and parathyroidectomy. A control group was sham operated. Various combinations in status of 1,25-(OH)2D and PTH were produced by daily sc injections of 1,25-(OH)2D (1.25) and/or continuous infusion of 1-34 bovine PTH. These were 1.25+/PTH+, 1.25-/PTH-, 1.25+/PTH-, 1.25-/PTH+. Serum calcium levels were kept in the normal range by the administration of one or two of the hormones or by oral supplementation of calcium lactate. Histomorphometric evaluation of static and dynamic parameters of bone after 8 months of experimental observation revealed that deficiency in 1,25-(OH)2D and PTH resulted in decreased number and activity of bone-forming and resorbing cells. Administration of 1,25-(OH)2D increased the activity but not the number of bone cells. In contrast, administration of PTH increased the number but not the activity of bone cells. Tissue level activity was decreased when one or both hormones were deficient, and normal tissue level activity was found only when both hormones were given. These data are relevant for understanding and management of diseases with perturbations in vitamin D and/or PTH.

AB - The singular and combined effects of 1,25-dihydroxyvitamin D [1,25-(OH)2D] and PTH on bone were evaluated in a long term in vivo study in dogs. Dogs were rendered deficient in 1,25-(OH)2D and PTH by five sixths nephrectomy and parathyroidectomy. A control group was sham operated. Various combinations in status of 1,25-(OH)2D and PTH were produced by daily sc injections of 1,25-(OH)2D (1.25) and/or continuous infusion of 1-34 bovine PTH. These were 1.25+/PTH+, 1.25-/PTH-, 1.25+/PTH-, 1.25-/PTH+. Serum calcium levels were kept in the normal range by the administration of one or two of the hormones or by oral supplementation of calcium lactate. Histomorphometric evaluation of static and dynamic parameters of bone after 8 months of experimental observation revealed that deficiency in 1,25-(OH)2D and PTH resulted in decreased number and activity of bone-forming and resorbing cells. Administration of 1,25-(OH)2D increased the activity but not the number of bone cells. In contrast, administration of PTH increased the number but not the activity of bone cells. Tissue level activity was decreased when one or both hormones were deficient, and normal tissue level activity was found only when both hormones were given. These data are relevant for understanding and management of diseases with perturbations in vitamin D and/or PTH.

UR - http://www.scopus.com/inward/record.url?scp=0022547275&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022547275&partnerID=8YFLogxK

M3 - Article

C2 - 3755395

AN - SCOPUS:0022547275

VL - 119

SP - 1298

EP - 1304

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 3

ER -