1α,25(OH)2D3 Regulation of integrin expression is substrate dependent

P. Raz, C. H. Lohmann, J. Turner, L. Wang, N. Poythress, C. Blanchard, B. D. Boyan, Z. Schwartz

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Osteoblasts are attachment-dependent cells that interact with their surface through integrin-mediated mechanisms. Their differentiation is regulated by 1,25-dihydroxyvitamin D3 [1α,25(OH)2D3] and is affected by substrate chemistry and microtopography, suggesting that 1α,25(OH)2D3 may regulate integrin expression in a surface-specific manner. To test this hypothesis, osteoblast-like human MG63 cells were grown on tissue culture plastic and on grit-blasted and acid-etched titanium disks with a complex microtopography to induce osteoblast differentiation. Expression of α2, α5, αv, β1, and β3 integrals were quantified by real-time polymerase chain reaction (PCR) as a function of time in culture and treatment with 1α,25(OH)2D3. Results were correlated with expression of osteocalcin, a marker of a differentiated osteoblast. Osteocalcin mRNA increased with time and 1α,25(OH) 2D3 treatment and these changes were greater in cultures on the titanium disks. Integrin expression varied with time in culture and this was also surface dependent. At each time point, β1 and α2 mRNAs were greater on titanium than on plastic, whereas α5 expression was reduced and αv, β3 expression was unaffected. 1α,25(OH)2D 3 increased β1 mRNA on both surfaces at all time points, but it increased α2 expression only in 8-d cultures. 1α,25(OH)2D3 caused reduced α5 expression only in cultures grown on plastic for 8 d, and had no effect on either αv or β3 expression regardless of surface. These results show that integrin expression in human osteoblast-like cells is differentially modulated by 1α,25(OH)2D3 in a time-dependent manner that is sensitive to the surface on which the cells are grown.

Original languageEnglish (US)
Pages (from-to)217-225
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume71
Issue number2
DOIs
StatePublished - Nov 1 2004

Keywords

  • 1α,25(OH)D
  • Integrins
  • Osteoblasts
  • Osteocalcin
  • Real-time polymerase chain reaction
  • Substrate surface
  • Titanium

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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    Raz, P., Lohmann, C. H., Turner, J., Wang, L., Poythress, N., Blanchard, C., Boyan, B. D., & Schwartz, Z. (2004). 1α,25(OH)2D3 Regulation of integrin expression is substrate dependent. Journal of Biomedical Materials Research - Part A, 71(2), 217-225. https://doi.org/10.1002/jbm.a.30134