β-Globin transcript found in induced murine erythroleukemia cells is homologous to the βho and βhl genes

B. A. Brown, R. W. Padgett, S. C. Hardies, C. A. Hutchison, M. H. Edgell

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Abstract

RNA transcripts complementary to at least one of the four β-globin homologous genes (βh) are found in high concentration in the murine erythroleukemic (MEL) cell line GM979 after butyric acid induction. Hybridization data indicate that the gene expressed is Hbb-βhO or Hbb-βh1, or both. The level of βhO/1 transcripts in the MEL cell is similar to the level of adult transcripts. The Hbb-βhO/1 transcript is about 800 nucleotides in length. In addition, there is a larger βhO/1 transcript of the same size and relative intensity as the adult β-globin precursor. We also report significant levels of embryonic gene Hbb-y transcripts in induced GM979 cells. We have determined that the GM979 cell line has the [Hbb](s) haplotype on the basis of an examination of its globin DNA restriction pattern. An additional MEL cell line of haplotype [Hbb](d) (DBA/2 line 6A11A) was examined and found to contain no significant level of Hbb-βhO, Hbb-βh1, Hbb-βh2, or Hbb-y gene transcripts.

Original languageEnglish (US)
Pages (from-to)2753-2757
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume79
Issue number9 I
DOIs
Publication statusPublished - Dec 1 1982

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