β-Adrenoceptors in the extraorbital lacrimal gland of the Syrian hamster characterization with [125I]-iodopindolol and evidence of sexual dimorphism

A. Pangerl, B. Pangerl, D. J. Jones, R. J. Reiter

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Saturation and competition experiments with the radiolabeled β-adrenergic antagonist (-)-[125I]-iodopindolol were used to characterize β-adrenoceptor density (Bmax) and receptor affinity in the extraorbital lacrimal gland of male and female Syrian hamsters. Specific binding to the receptor was saturable. Scatchard analysis of saturation isotherms revealed a single population of receptor sites. Male glands had a significantly higher Bmax (38.9±5.0 vs. 23.3±2.1 fmole/mg protein, -x±SEM, p< 0.02) and receptor affinity (expressed in a lower dissociation constant Kd: 0.065±0.013 vs. 0.120±0.015 nM, p<0.02) than female glands. Binding of the radiolabeled ligand in competition with various adrenergic antagonists showed the receptor to be stereospecific and of the β2-subtype.

Original languageEnglish (US)
Pages (from-to)153-162
Number of pages10
JournalJournal of Neural Transmission
Volume77
Issue number2-3
DOIs
StatePublished - Jun 1 1989

Keywords

  • [I]-iodopindolol
  • lacrimal gland
  • melatonin
  • receptor binding
  • sexual dimorphism
  • β-Adrenoceptor

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Psychiatry and Mental health
  • Biological Psychiatry

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