β-Adrenoceptors in the extraorbital lacrimal gland of the Syrian hamster characterization with [125I]-iodopindolol and evidence of sexual dimorphism

A. Pangerl, B. Pangerl, D. J. Jones, Russel J Reiter

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Saturation and competition experiments with the radiolabeled β-adrenergic antagonist (-)-[125I]-iodopindolol were used to characterize β-adrenoceptor density (Bmax) and receptor affinity in the extraorbital lacrimal gland of male and female Syrian hamsters. Specific binding to the receptor was saturable. Scatchard analysis of saturation isotherms revealed a single population of receptor sites. Male glands had a significantly higher Bmax (38.9±5.0 vs. 23.3±2.1 fmole/mg protein, -x±SEM, p< 0.02) and receptor affinity (expressed in a lower dissociation constant Kd: 0.065±0.013 vs. 0.120±0.015 nM, p<0.02) than female glands. Binding of the radiolabeled ligand in competition with various adrenergic antagonists showed the receptor to be stereospecific and of the β2-subtype.

Original languageEnglish (US)
Pages (from-to)153-162
Number of pages10
JournalJournal of Neural Transmission
Volume77
Issue number2-3
DOIs
StatePublished - Jun 1989

Fingerprint

Lacrimal Apparatus
Adrenergic Antagonists
Mesocricetus
Sex Characteristics
Adrenergic Receptors
Ligands
Population
Proteins

Keywords

  • β-Adrenoceptor
  • [I]-iodopindolol
  • lacrimal gland
  • melatonin
  • receptor binding
  • sexual dimorphism

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

β-Adrenoceptors in the extraorbital lacrimal gland of the Syrian hamster characterization with [125I]-iodopindolol and evidence of sexual dimorphism. / Pangerl, A.; Pangerl, B.; Jones, D. J.; Reiter, Russel J.

In: Journal of Neural Transmission, Vol. 77, No. 2-3, 06.1989, p. 153-162.

Research output: Contribution to journalArticle

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