α5 Integrin subunit expression changes during myogenesis

Bjorn Steffensen, Victoria L. Magnuson, Cheryl L. Potempa, Dali Chen, Robert J. Klebe

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Fibronectin and its cellular receptor, the α5β1 integrin, are involved in the transmembrane signalling events that control muscle cell differentiation. In this study, the expression of the α5 integrin subunit was followed by reverse transcription-polymerase chain reaction (RT-PCR) to determine alterations during myogenesis. In studies of murine muscle, we found a 90% reduction in the level of the α5 integrin subunit mRNA during early postnatal development. Concurrently, the fibronectin alternative splicing pattern changed markedly in the EIIIB and V exons. In-vitro analyses of these molecules during myoblast differentiation revealed changes that followed trends similar to those observed in vivo, although of lesser magnitude. These observations imply an important role of fibronectin and the α5 integrin subunit in muscle development.

Original languageEnglish (US)
Pages (from-to)95-100
Number of pages6
JournalBBA - Molecular Cell Research
Volume1137
Issue number1
DOIs
StatePublished - Oct 6 1992

Keywords

  • Cell differentiation
  • Fibronectin
  • Fibronectin alternative splicing
  • Integrin
  • mRNA
  • Muscle
  • Myogenesis
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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