α(1B) adrenoceptors in rat paraventricular nucleus overlap with, but do not mediate, the induction of c-Fos expression by osmotic or restraint stress

A. M. Williams, David A Morilak

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A role has been suggested for hypothalamic α1 adrenoceptors in the acute stress-induced activation of the hypothalamic-pituitary-adrenal axis. Using a polyclonal antiserum against the rat α(1B) adrenergic receptor protein, we have demonstrated α(1B) receptor immunoreactivity in neurons and especially in punctate cell processes in the rat paraventricular nucleus. The distribution of α(1B) receptor immunoreactivity overlapped in part with the distributions of c-Fos immunoreactivity induced in the paraventricular nucleus by either restraint stress or hypertonic saline administration. However, intraperitoneal pretreatment with the α1 receptor antagonist prazosin (0.5 or 5.0 mg/kg) failed to attenuate stress-induced c-Fos expression in the paraventricular nucleus. Prazosin also failed to attenuate the secretion of corticosterone following restraint stress. Thus, we conclude that neither acute secretory activity nor activation of gene transcriptional responses mediated by c-Fos in the hypothalamic-pituitary-adrenal axis following these stressors are dependent upon hypothalamic α1 adrenergic receptors.

Original languageEnglish (US)
Pages (from-to)901-913
Number of pages13
JournalNeuroscience
Volume76
Issue number3
DOIs
StatePublished - Dec 11 1996

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Paraventricular Hypothalamic Nucleus
Adrenergic Receptors
Prazosin
Transcriptional Activation
Osmotic Pressure
Corticosterone
Immune Sera
Neurons
Proteins

Keywords

  • α adrenergic receptors
  • c-Fos
  • HPA axis
  • paraventricular nucleus
  • stress

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "α(1B) adrenoceptors in rat paraventricular nucleus overlap with, but do not mediate, the induction of c-Fos expression by osmotic or restraint stress",
abstract = "A role has been suggested for hypothalamic α1 adrenoceptors in the acute stress-induced activation of the hypothalamic-pituitary-adrenal axis. Using a polyclonal antiserum against the rat α(1B) adrenergic receptor protein, we have demonstrated α(1B) receptor immunoreactivity in neurons and especially in punctate cell processes in the rat paraventricular nucleus. The distribution of α(1B) receptor immunoreactivity overlapped in part with the distributions of c-Fos immunoreactivity induced in the paraventricular nucleus by either restraint stress or hypertonic saline administration. However, intraperitoneal pretreatment with the α1 receptor antagonist prazosin (0.5 or 5.0 mg/kg) failed to attenuate stress-induced c-Fos expression in the paraventricular nucleus. Prazosin also failed to attenuate the secretion of corticosterone following restraint stress. Thus, we conclude that neither acute secretory activity nor activation of gene transcriptional responses mediated by c-Fos in the hypothalamic-pituitary-adrenal axis following these stressors are dependent upon hypothalamic α1 adrenergic receptors.",
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AU - Williams, A. M.

AU - Morilak, David A

PY - 1996/12/11

Y1 - 1996/12/11

N2 - A role has been suggested for hypothalamic α1 adrenoceptors in the acute stress-induced activation of the hypothalamic-pituitary-adrenal axis. Using a polyclonal antiserum against the rat α(1B) adrenergic receptor protein, we have demonstrated α(1B) receptor immunoreactivity in neurons and especially in punctate cell processes in the rat paraventricular nucleus. The distribution of α(1B) receptor immunoreactivity overlapped in part with the distributions of c-Fos immunoreactivity induced in the paraventricular nucleus by either restraint stress or hypertonic saline administration. However, intraperitoneal pretreatment with the α1 receptor antagonist prazosin (0.5 or 5.0 mg/kg) failed to attenuate stress-induced c-Fos expression in the paraventricular nucleus. Prazosin also failed to attenuate the secretion of corticosterone following restraint stress. Thus, we conclude that neither acute secretory activity nor activation of gene transcriptional responses mediated by c-Fos in the hypothalamic-pituitary-adrenal axis following these stressors are dependent upon hypothalamic α1 adrenergic receptors.

AB - A role has been suggested for hypothalamic α1 adrenoceptors in the acute stress-induced activation of the hypothalamic-pituitary-adrenal axis. Using a polyclonal antiserum against the rat α(1B) adrenergic receptor protein, we have demonstrated α(1B) receptor immunoreactivity in neurons and especially in punctate cell processes in the rat paraventricular nucleus. The distribution of α(1B) receptor immunoreactivity overlapped in part with the distributions of c-Fos immunoreactivity induced in the paraventricular nucleus by either restraint stress or hypertonic saline administration. However, intraperitoneal pretreatment with the α1 receptor antagonist prazosin (0.5 or 5.0 mg/kg) failed to attenuate stress-induced c-Fos expression in the paraventricular nucleus. Prazosin also failed to attenuate the secretion of corticosterone following restraint stress. Thus, we conclude that neither acute secretory activity nor activation of gene transcriptional responses mediated by c-Fos in the hypothalamic-pituitary-adrenal axis following these stressors are dependent upon hypothalamic α1 adrenergic receptors.

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