• Valente, Anthony J (PI)

Project: Research project

Project Details


The overall aim of this project is to fully characterize the monocyte
chemoattractant SMC-CF, which is secreted by baboon aortic smooth muscle
cells (SMC) and which may regulate the intimal recruitment of blood
monocytes in atherogenesis. This potent chemoattractant is associated with
a low molecular weight (12-16 kD) basic monomeric protein which has been
successfully purified to homogeneity. Baboon SMC express the c-sis gene,
which encodes one of the two polypeptide chains of platelet-derived growth
factor (PDGF), a potent mitogen for SMC. PDGF in both its monomeric and
dimeric forms is also a potent chemoattractant for monocytes. Preliminary
metabolic labeling studies indicate that baboon SMC synthesize and secrete
non-mitogenic, monomeric PDGF-like proteins of MW 100 kD and 12 kD. It is
possible that SMC-CF and PDGF-like protein are related. The aim of these
studies is to determine the primary amino acid sequence of SMC-CF, by
determining the nucleotide sequencing of SMC-CF cDNA and compare it with
the published sequence of PDGF and other proteins for homology. A
polyclonal antiserum to SMC-CF will be developed for use in determining
structural similarity between SMC-CF and PDGF and other chemoattractants.
In addition, antibodies to PDGF and PDGF-related peptides provided by Drs.
Antoniades and Graves will be used. Receptor studies will be carried out
to characterize the binding of SMC-CF to monocytes. The functional
similarity between SMC-CF, PDGF and other chemoattractants will be
determined by competition for receptor sites. The intracellular processing
pathways of both SMC-CF and PDGF-like proteins will be established in the
SMC utilizing immunoprecipitation techniques. The influence of SMC
phenotype on SMC-CF secretion, c-sis expression and the synthesis of
PDGF-like proteins will also be examined. These proposed studies will
provide basic biologic insights into the mechanism whereby SMC might
regulate monocyte recruitment to the intima in atherogenesis.
Effective start/end date4/1/877/31/90


  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health


  • Medicine(all)


Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.