• Tardif, Suzette D (PI)
  • Legler, Donald W. (PI)
  • Zimmerman, Stuart O. (PI)
  • Bowers, Larry (PI)
  • Dukelow, W. Richard (PI)
  • Sullivan, Louis W. (PI)
  • Kier, Ann B. (PI)
  • Toth, Linda (PI)
  • Ziegler, Michael G. (PI)
  • Schlechte, Janet A. (PI)

Project: Research project

Project Details


Funds are requested for the purchase of an atmospheric pressure ionization
(API) tandem quadrupole mass spectrometer (MS/MS) for use in biomedical and
other research projects at the University of Minnesota Health Sciences
complex. Recent advances in "soft ionization techniques" including the
electrospray and ion-spray API methods have made mass spectrometry an
extremely important tool in biomedical research. When the ability to
produce a molecular ion is coupled with collisionally-induced dissociation
(CID), both molecular weight and structural information are available from
a single experiment. These capabilities have been well utilized for
determination of amino acid sequences of peptides, sequences in
oligonucleotides, and structural information about polysaccharides. API
methods have been long recognized for their excellent sensitivity, a fact
of key importance for producing quantitative data on limited amounts of
biological fluids. The proposed research projects make use of the
sensitivity of API, the excellent limits of detection of selected reaction
monitoring, and the structural elucidation capability of MS/MS as well as
the extended mass range (2400 amu) and negative ionization capabilities not
available on present Health Sciences instrumentation. The proposed instrument will support a primary users group of seven
investigators representing six departments within the Health Sciences. The
research projects include linear and cyclic peptide sequencing for
immunosuppressive drugs, qualitative and quantitative studies on
neurochemically active amino acid analogs and peptides, identification of
nucleotide regulatory compounds, quantitative metabolic studies on a
nucleotide analog effective against AIDS, and identification of immuno-
inhibitory components of mannans from microbes. The requested instrument
will join a Hewlett-Packard 5985A GC/MS and a VG Sira II IDMS to provide a
Health Sciences Mass Spectrometry Resource. All of the instrumentation in
the Resource will be available to any researcher, and operation will be
financed by a users fee. The MS/MS will be employed solely for research
studies, using soft ionization techniques as well as HPLC/MS/MS and
capillary zone electrophoresis/MS/MS. In addition to the primary
investigators, faculty utilizing a program project grant laboratory and the
molecular biology laboratory will be encouraged to use this instrument.
Education of these potential users will be a major goal of the shared
facility. The requested instrument will provide the Health Sciences Mass
Spectrometry Resource with a state-of-the-art instrument to respond to the
diverse requests inherent to such a facility and complement other mass
spectrometers available at the University of Minnesota.
Effective start/end date7/1/849/30/05


  • National Institutes of Health: $400,000.00
  • National Institutes of Health: $780,000.00


  • Medicine(all)


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