Project: Research project

Project Details


The goal of this program project is to produce detailed physical and
genetic maps of polymorphic markers for human chromosomes 2 and 3. The
current maps of chromosomes 2 and 3 only have a limited number of markers
and there are several large gaps on both the physical and genetic maps.
The physical map generated by the program project will be based on
radiation reduction hybrids and the genetic map a result of analyzing CEPH
family DNA. The genetic markers generated will all be PCR based and
consist of highly polymorphic markers such as found with simple di-, tri-,
and tetranucleotide repeats and in the poly A tract of L1 sequences as well
as transcribed sequences specific for these chromosomes. In addition the
program will devise a method for globally mapping each chromosome with Line
elements and correlating this map to the genetic and radiation hybrid map.
To accomplish these goals, the program consists of 4 projects and a core
for linkage studies as well as an administrative core. These highly
interdependent projects will result in the placement of 600 highly useful
markers on chromosomes 2 and 3 over the grant period. The markers are
termed highly useful since they will be highly polymorphic, be based on PCR
and available to all investigators, and be mapped on both physical and
genetic maps. The physical map generated will be complete at the 2 cM
level and in many regions 1 cM distances will be achieved. The physical
map of each chromosome will have approximately 1 marker per megabase. This
framework panel of markers and cell reagents will be used to direct the
isolation of ordered clones for chromosomes 2 and 3 and will be especially
useful for closure on these chromosomes. The polymorphic markers will be
immediately applicable for identifying markers adjacent to disease loci.
Effective start/end date6/10/925/31/98


  • National Institutes of Health


  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)


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