REGULATION OF NEUROPEPTIDE RELEASE FROM DENTAL PULP

Project: Research project

Project Details

Description

Although neuropeptides are contained within primary afferent fibers
located in dental pulp, the pharmacological regulation of their release
has been incompletely characterized. This area of research has been
limited by access, since dental pulp is encased in a hard structure
similar to the encasement of the spinal cord within the vertebral column.
However, recent studies have been successfully carried out with in vitro
superfusion of spinal cord preparations. These studies have determined,
to a large extent, the pharmacological regulation of neuropeptide release
from spinal cord tissue. Similarly, this proposal will employ an in
vitro superfusion of dental pulp to accomplish the following specific
aims: 1) Compare capsaicin to potassium for evoking release of immunoreactive
substance P (iSP) and calcitonin gene-related peptide (iCGRP) from dental
pulp using an in vitro superfusion preparation. 2) Characterize the physiologic mechanisms mediating pulpal secretion of
neuropeptides. 3) Determine whether bradykinin and prostaglandin E2 (PGE2) are effective
for evoking the release of iSP and iCGRP from dental pulp. 4) Determine whether adrenergic agonists selective for the alpha and beta
receptor subtypes are effective in modulating basal or evoked-release of
iSP and iCGRP from dental pulp superfusates. Our initial data demonstrates the feasibility of this approach.
Administration of a potassium pulse to a superfused dental pulp
preparation elicits a near six-fold increase in iSP and a near two-fold
increase in iCGRP as compared to basal levels of release. This method
appears suitable for more comprehensive studies determining the
pharmacological regulation of neuropeptide release from dental pulp. Development of an in vitro model to evaluate these regulatory mechanisms
may prove useful in studies on the physiology of primary afferent fibers
mediating neurogenic inflammation and may serve to identify potentially
useful therapeutic interventions.
StatusFinished
Effective start/end date9/30/929/29/94

Funding

  • National Institutes of Health
  • National Institutes of Health: $34,409.00

ASJC

  • Medicine(all)
  • Dentistry(all)

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