PATHOBIOLOGY OF CULTURED GLOMERULAR EPITHELIAL CELLS

  • Kasinath, Balakuntalam S (PI)

Project: Research project

Project Details

Description

The aim of this project is to study the response of cultured
glomerular visceral epithelial cells to nonimmunologic and
immunologic injury. Glomerular epithelial cells appear to play a
role in puromycin aminonucleoside nephrosis and Heymann
nephritis in rat. These experimental models closely resemble
human glomerular diseases - minimal change nephrotic syndrome
and membranous nephropathy, respectively. We have previously
shown that these receptors bear receptors for C3, antigens of
Heymann nephritis on their surface and that they synthesize
heparan sulfate proteoglycan. Sialic acid residues, richly
deposited on these epithelial cells, and, glomerular heparan
sulfate are said to be involved in glomerular barrier function
against protein loss. Complement system including the membrane
attack complex is known to be prominently involved in
pathogenesis of many glomerular diseases including Heymann
nephritis. Employing the above properties, specifically, we
propose to study the following: I. Effect of puromycin aminonucleoside (nonimmunologic injury)
on Heymann nephritis related antigen, heparan sulfate and sialic
acid contents of glomerular epithelial cells. II. A. Effect of binding of antibody to Heymann nephritis related
antigen located on glomerular epithelial cells (immunologic injury)
on expression of C3 receptors, content of heparan sulfate and
sialic acid.
B. Effects of binding of C3 ligands, membrane attack
complex, alone or in combination (immunologic injury) on content
of heparan sulfate and sialic acid. Methods employed will consist of cell culture of glomerular
epithelial cells, quantitative immunoperoxidase, standard rosette
techniques using sheep erythrocytes as indicator particles. In
immunoperoxidase reactions, highly purified, well described
specific antibodies will be used. Standard parametric and
nonparametric statistical methods will be used in analysis of
results. Our results will advance understanding the role of
glomerular visceral epithelial cells in the two experimental renal
disease models which are prototypes of common human
glomerulopathies.
StatusFinished
Effective start/end date12/1/8611/30/89

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

ASJC

  • Medicine(all)

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