Project: Research project

Project Details


This proposal is designed to study the biologic mechanisms involved in
normal and abnormal development of craniofacial sutures. The methods to be
used include cleared alizarin preparations, specimen radiography, light,
electron and immunofluorescent microscopy and biochemical analyses for
fibronectin, proteoglycans, and the different types of collagen in the
sutural ligaments. The material to be studied consists of a collection of
timed prenatal Macaca nemestrina specimens, normal and abnormal genetic
strains of mice (wild type C57B1/6J, brachiomorphic bm/bm, and viable
brindled MOvbr), rats and rabbits. The specific aims are: (1) to study
histologic ultrastructural and biochemical differentiation of normal and
genetically abnormal sutures in vivo, (2) to develop an in vitro system
that will allow the application of stretching and compressive forces to
developing sutures in tissue culture, and (3) to produce immature sutures
in young calvariectomized rabbits for experiments on premature sutural
fusion. The hypothesis to be tested is that sutural synostosis can be
caused by intrauterine compression of immature cranial sutures in contrast
to the sutural remodeling that results when mature sutures are compressed
postnatally. If this clinical hypothesis is confirmed experimentally, it
will provide a pathogenetic mechanism for this presently unexplained
Effective start/end date8/1/81 → …


  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health


  • Medicine(all)
  • Dentistry(all)


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