Project: Research project

Project Details


This grant proposal focuses on four distinct yet interrelated
aspects of the molecular pathogenesis of human mycoplasma
disease, emphasizing surface membrane properties of Mycoplasma
pneumoniae and Mycoplasma genitalium. The first describes our
attempts to understand cytadherence by characterizing the
mycoplasma adhesins implicated in host cell parasitism. Various
techniques will be employed including column chromatography,
amino acid sequencing, oligonucleotide determinations,
recombinant DNA methodology, mutant analysis, and gene
transfer systems. The second focuses on specific lactoferrin-binding membrane
components (receptors) of M. pneumoniae and their possible role
in tissue colonization and/or virulence. These are recent
observations, and data suggest the potentially important role of
the lactoferrin-M. pneumoniae interaction in the metabolic
integrity of these pathogens. The third will utilize a variety of already generated (and to be
generated) immunological probes to study ultrastructural
relationships among the mycoplasma adhesins and lactoferrin-
binding membrane components by immunoelectron microscopic
analysis using antibody-colloidal gold reagents. The fourth attempts of develop models for assessing virulence of
M. genitalium. The current dependence on humans and non-human
primates severely restricts virulence testing of wild-type strains
and potential avirulent mutants of M. genitalium.
Effective start/end date12/1/8311/30/90


  • National Institutes of Health


  • Medicine(all)
  • Immunology and Microbiology(all)


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