MOLECULAR ANALYSIS OF ADHESIN P1

Project: Research project

Project Details

Description

This research proposal is focused on the significance of multiple gene
copies for protein P1 with regards to the observed biological role of P1
as an integral adhesin necessary for cytadherence of Mycoplasma
pneumoniae to human respiratory epithelium. Emphasis is place on
defining at the molecular level the epitopes within P1 necessary for
cytadherence and detecting antigenic or biological alterations in these
epitopes in wild-type, mutant and clinical isolate populations. The
definition of functional epitopes within P1 and the identification of
unique-sequences form P1-related gene fragments will be used to explore
the factors which affects the expression and biological role of adhesin
P1 in virulence. Technologies to be used range from cloning and
sequencing of P1 and P1-related gene sequences to analysis of mRNA
transcripts and development of expression systems for M. pneumoniae
proteins. In addition, synthetic peptides will be examined from vaccine
and serodiagnostic value and will assist in characterization of host
receptors. We expect that other activities of P1 will be uncovered in
addition to those related to cytadherence. It is expected that information obtained from this study will result in a
significantly better molecular understanding of the virulence process of
Mycoplasma pneumoniae. This understanding will ultimately lead to
interventions for the prevention of pneumonia cause by M. pneumoniae and
related infections. More generally, these studies will further the
understanding of the role of gene conversion in the alteration of protein
domains with biofunctional significance.
StatusFinished
Effective start/end date9/1/897/31/95

Funding

  • National Institutes of Health
  • National Institutes of Health: $210,715.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)

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