Project: Research project

Project Details


The Mass Spectrometry Laboratory of the University of Texas Health
Science Center at San Antonio functions as a multi-user support
service for the Health Science Center and for the Southwest
Research Consortium. Investigators from these institutions,
involved in many different areas of biomedical research, utilize
this facility. Currently, there are two Hewlett-Packard 5982
quadrupoles mass spectrometers and a Finnigan-MAT 212 magnetic
sector instrument. One of the quadrupoles is used for direct
insertion probe EI samples and packed column GC-MS analyses. The
other quadrupole has been modified for thermospray HPLC-MS. The
magnetic sector instrument is used primarily for FAB and non-
routine capillary GC-MS (EI or CI). Less frequently, DCI and HPLC-
MS (belt interface) are performed. quadrupole mass spectrometers
were manufactured in 1975 and are now obsolete, both in the nature
of their design and in the availability of parts and service.
Hewlett-Packard no longer offers service contracts on these units,
and many of the parts are not being made anymore. A new mass
spectrometer is needed at this institution to: 1) assume the
routine analyses currently being performed on the Hewlett-Packard
quadrupole; 2) provide the capability for chemical ionization with
negative ion detection. The first function will preserve the
current status and is essential for projects requiring EI-GC-MS
and direct insertion probe analyses. The second function will
provide access to a technique of superior sensitivity and
selectivity, which is critical to facilitate further progress in
numerous ongoing investigations. A secondary aspect of this is proposal is upgrading the data system
on the magnetic sector instrument. The current arrangement for
data storage, which includes a 5 mb fixed disk and 5 mb removable
cartridges, is restrictive in the number of spectra which can be
preserved and is both unwieldy and laborious to use for file
manipulation. Furthermore, the Nova 4C computer cannot acquire
data at a fast enough rate to perform medium resolution capillary
GC-MS. It is, therefore, essential that a new quadrupole mass spectrometer
be obtained to perform daily service analyses and to provide highly
sensitive NCI measurements. The purchase of a new quadrupole,
along with upgrading of the data system on the magnetic sector
instrument, will significantly enhance the probability for success
of a multitude of research projects at this institution
Effective start/end date12/1/8711/30/88


  • National Institutes of Health


  • Medicine(all)


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