FETAL NEUROENDOCRINOLOGY, PARTURITION AND THE MYOMETRIUM

  • Nijland, Mark J (PI)
  • Cox, Laura, (PI)
  • Jansson, Thomas (PI)
  • Poore, E. Robin (PI)
  • Reimers, Thomas (PI)
  • Wen Xuan, Wu (PI)
  • Weintraub, Susan (PI)
  • Myers, Dean (PI)
  • Meyers, Dean (PI)
  • Wentworth, Richard (PI)
  • McDonald, Thomas (PI)
  • Nathanielsz, Peter (PI)
  • Weintraub, Susan E (PI)

Project: Research project

Description

The three integrated projects test fundamental hypotheses related
to fetal neuro-endocrinology, parturition and regulation of the
myometrium in parallel experiments in chronically instrumented
pregnant sheep and pregnant rhesus monkey preparations. The
Reproductive Studies Research Group at Cornell has developed
the animal techniques, computerized biophysical systems and
biochemical and endocrinological methods required to study these
two experimental models. We propose a multidisciplinary
approach in which selected single variables will be changed with
appropriate statistical analysis. Project 1 continues seven years of NIH funded work to study
initiation and completion of parturition in sheep. It tests the
hypothesis that both fetal and maternal neural and hormonal
mechanisms regulate epochs of chronic long-lasting myometrial
activity and contractures, throughout gestation and that one or
more of these factors regulate the change of contractures to
labor and delivery contractions. It examines regulation of
contractures and contractions by arachidonic acid metabolites,
steroids, uterine stretch and starvation. Projects 1 and 2 examine the fetal hypothalamo-hypophyseal-
adrenal axis (HHAA) using immunocytochemical techniques and
stereotaxic lesions of the fetal hypothalamus. They focus on
histological development of oCRF containing neurons and the
fetal PVN's role in pulsatile secretion of fetal ACTH. They will
evaluate effects of fetal PVN lesions on glucocorticoid feedback
and secretion of ACTH in response to stress stimuli, and will
determine if PVN oCRF-containing neurons are involved in the
increased activity of the fetal HHAA which may play a central
role in initiation of parturition. Project 3 examines similar systems in the rhesus monkey. We will
develop the capability to lesion the PVN and place lateral
ventricular catheters in fetal rhesus monkeys at 120 days
gestation to study the role of CRF and AVP in regulation of the
fetal rhesus HHAA. Control of the primate myometrium will be
investigated both in vitro and in vivo. We will undertake
immunocytochemistry of the fetal hypothalamus. Prematurity and complications of delivery are major obstetric
problems and increase perinatal mortality and morbidity. These
experiments will define similarities and differences between
sheep and non-human primates.
StatusFinished
Effective start/end date12/1/861/31/19

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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Papio
Kidney
Food
Mothers
Epigenomics
Bioengineering
Primates
Proteomics
Genomics
Macaca mulatta
Intercellular Signaling Peptides and Proteins
Pregnancy
Fetal Development
Parturition
Contracture
Adrenocorticotropic Hormone
Hypothalamus
Growth
Sheep
Renin-Angiotensin System

ASJC

  • Medicine(all)