EFFICIENT DELIVERY OF HIV-REGULATED TOXIN GENES FOR AIDS

Project: Research project

Project Details

Description

The overall objective for this NCDDG application is to evaluate different
methods for efficient delivery to toxin genes as a gene therapy approach
for Acquired Immune Deficiency Syndrome (AIDS). Plasmid and retroviral
constructs were previously generated from which toxin gene expression was
controlled by HIV-1 regulatory proteins Tat and Rev. Stable cell lines
were derived which had been transfected or infected with DT-A-encoding
plasmids or retroviruses, respectively. Such cell lines were markedly
impaired in their ability to be infected by laboratory to improve the
efficiency of transfection of the toxin gene.
(Project 9001) will serve as an animal core, to
evaluate the in vivo anti-HIV effectiveness of toxin genes, delivered by
methods described in Projects 1-2. The experiments described here should
lead directly to preclinical safety and toxicity studies for HIV-
regulated toxin genes. Ultimately, we hope HIV regulated toxin gene
expression can be developed as a therapy for AIDS, where the immune
system would be reconstituted with self-renewing, immune-competent cells
which are resistant to HIV infection.
StatusFinished
Effective start/end date1/1/9412/31/96

Funding

  • National Institutes of Health
  • National Institutes of Health

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)

Fingerprint Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.