EFFICACY STUDIES OF CHEMOPREVENTIVE AGENTS

Project: Research project

Description

The efficacy of a variety of chemopreventive agents to reduce or inhibit colon cancer using male Fisher 344 rats treated with a carcinogen +/- chemopreventive agents is being assessed. Aberrant crypt formation four weeks after the first carcinogen exposure is being measured. All rats, approximately 8 weeks old, are treated with two injections of 15 mg of azoxymethane (AOM) per kg of body weight one week apart. An dose selection study is performed prior to the chemoprevention study for each agent at five doses. For Protocol A: Administer each agent at five doses to approximately seven-week-old rats. Feed the rats the test chemopreventive agent from one week prior to and four weeks following the first carcinogen treatment. Then kill the rats and score the aberrant crypts. For Protocol B: Administration of the chemopreventive agent shall begin 4 weeks after the first carcinogen dose and continued for four weeks. At eight weeks following the first carcinogen treatment aberrant crypts will be scored. For both protocols, the number of aberrant crypt foci per colon and the number of aberrant crypts per foci from unsectioned methylene-blue stained colons are being determined. The two doses to be used in the aberrant crypt foci study are determined from data produced in the rangefinding assay and obtain approval from the Project Officer. Ten animals are being used per group. There are 20 agents in protocol A and 10 in protocol B.
StatusActive
Effective start/end date9/30/95 → …

Funding

  • National Institutes of Health
  • National Institutes of Health

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Carcinogens
Aberrant Crypt Foci
Colon
Azoxymethane
Methylene Blue
Chemoprevention
Colonic Neoplasms
Body Weight
Injections
Therapeutics

ASJC

  • Medicine(all)