ANTIGEN IMMUNODIAGNOSIS OF MYCOTIC INFECTION

Project: Research project

Project Details

Description

The premise of these studies is that detection of candidal antigen(s) in
clinical body specimens will facilitate early diagnosis of invasive
candidiasis. Specific objectives are: 1. To rigorously evaluate the utility of 3 RIAs to candidal mannan-proteins
in prospective studies of granulocytopenic patients. The utility of a
mannan RIA with wide serotypic cross-reactivity will be compared to 2 RIAs
of greater serotypic specificity; to the detection of IgM and IgG
anticandidal antibodies; and to routine diagnostic procedures. In subgroup
analysis, RIAs will be compared to simpler immunoassay procedures
(objective 2), to new antigen probes (objective 3), and to GLC assays. 2. To improve the mannan-protein assays for immunodiagnosis by: Developing
rapid one-step, two-site antigen detection assays with available polyclonal
antisera and with monoclonal antibodies; adapting our competitive
inhibition RIAs to simple ELISA and our pilot agglutination assays for
gneral serology laboratiries; developing a simpler, more efficient
extraction for mannan-proteins from serum and urine by solid-phase
adsorbtion; developing high avidity monoclonal antibodies for use in RIAs
and other assays, and for use in novel diagnostic probes (objectives 3,4). 3. To identify by novel immunohistochemical, immunochemical, and
biochemical techniques, new antigen probes for immunodiagnosis of invasive
candidiasis. We propose to directly identify nonmannan antigens and
different epitopes of mannan-proteins elaborated in vivo during human
infection by this method and develop new probes to them. In addition, we
will identify by Western blotting the candidal antigens which are
predominant immunogens during invasive infection; correlated these
immunogens with the immunohistochemistry, and develop assays to immunogens
which appear likely to have diagnostic utility. 4. To use experimental models of candidasis to evaluate new diagnostic
techniques: endocarditis and meningitis for in vivo scintography of
radiolabeled candidal antibodies and for flow cytometry (FACS) of
phagocytes; pneumonia for immunoperoxidase cytology of bronchoalveolar
lavage cells; and kidney and bladder candidiasis to characterize mannan
antigenuria.
StatusFinished
Effective start/end date1/1/776/30/88

Funding

  • National Institutes of Health
  • National Institutes of Health

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)

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