ANTI-IDIOTYPE VACCINE DEVELOPMENT FOR SCHISTOSOMIASIS

  • Loverde, Philip T (PI)

Project: Research project

Project Details

Description

The overall goal of this research is to develop a vaccine against
schistosomiasis using novel approaches based on anti-idiotype antibodies
and synthetic peptide synthesis. The concept for these studies on the function, structure and therapeutic
use of 'Idiotope Vaccines' is based on the Immune Network Theory which
predicts the existence of so-called 'Internal Antigens'. Experimental
support for 'Internal Antigen' derived vaccines has been recently obtained
by one of us (HK) by inducing PC anti-streptococcal immunity using a
modified anti-idiotypic hybridoma. In the murine model of schistosomiasis, we will identify anti-idiotype
antibodies (internal antigens) and demonstrate their protective value
against infection. Then to make this applicable to humans we will identify
the internal antigen epitopes. This will be accomplished by deducing the amino acid sequence of the
variable regions of internal image anti-idiotypes by primer extension
sequencing of mRNA. Idiotopic regions expressing the internal antigen will
then be identified by computer modeling. Computer modeling studies will
indicate the synthetic peptides to be made. By immunizing mice with the
synthesized idiotope peptide-protein complex and subsequently challenging
them with Schistosoma we will demonstrate the feasibility of making a
synthetic idiotope vaccine. These studies will lay the ground work for specific vaccines against the
human schistosomes.
StatusFinished
Effective start/end date9/1/853/31/89

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)

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